Quantification of specific E. coli in gut mucosa from Crohn's disease patients
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
We here present a method based on qRT-PCR to quantify E. coli LF82 in intestinal human samples. Two different primer-probe sets were designed to detect LF82, and a third to target total E. coli. The assay showed high robustness and specificity for detection of LF82 in the presence of intestinal tissue.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Microbiological Methods |
Vol/bind | 86 |
Udgave nummer | 1 |
Sider (fra-til) | 111-4 |
Antal sider | 4 |
ISSN | 0167-7012 |
DOI | |
Status | Udgivet - jul. 2011 |
ID: 166456201