IMP2 axonal localization, RNA interactome, and function in the development of axon trajectories
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IMP2 axonal localization, RNA interactome, and function in the development of axon trajectories. / Preitner, Nicolas; Quan, Jie; Li, Xinmin; Nielsen, Finn C.; Flanagan, John G.
I: Development, Bind 143, Nr. 15, 2016, s. 2753-2759.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - IMP2 axonal localization, RNA interactome, and function in the development of axon trajectories
AU - Preitner, Nicolas
AU - Quan, Jie
AU - Li, Xinmin
AU - Nielsen, Finn C.
AU - Flanagan, John G.
PY - 2016
Y1 - 2016
N2 - RNA-based regulatory mechanisms play important roles in the development and plasticity of neural circuits and neurological disease. Developing axons provide a model well suited to the study of RNA-based regulation, and contain specific subsets of mRNAsthat are locally translated and have roles in axon pathfinding. However, the RNA-binding proteins involved in axon pathfinding, and their corresponding mRNA targets, are still largely unknown. Here we find that the RNA-binding protein IMP2 (Igf2bp2) is strikingly enriched in developing axon tracts, including in spinal commissural axons. We used the HITS-CLIP approach to perform a genome-wide identification of RNAs that interact directly with IMP2 in the native context of developing mouse brain. This IMP2 interactomewas highly enriched for mRNA targets related to axon guidance. Accordingly, IMP2 knockdown in the developing spinal cord led to strong defects in commissural axon trajectories at the midline intermediate target. These results reveal a highly distinctive axonal enrichment of IMP2, show that it interacts with a network of axon guidance-related mRNAs, and reveal that it is required for normal axon pathfinding during vertebrate development.
AB - RNA-based regulatory mechanisms play important roles in the development and plasticity of neural circuits and neurological disease. Developing axons provide a model well suited to the study of RNA-based regulation, and contain specific subsets of mRNAsthat are locally translated and have roles in axon pathfinding. However, the RNA-binding proteins involved in axon pathfinding, and their corresponding mRNA targets, are still largely unknown. Here we find that the RNA-binding protein IMP2 (Igf2bp2) is strikingly enriched in developing axon tracts, including in spinal commissural axons. We used the HITS-CLIP approach to perform a genome-wide identification of RNAs that interact directly with IMP2 in the native context of developing mouse brain. This IMP2 interactomewas highly enriched for mRNA targets related to axon guidance. Accordingly, IMP2 knockdown in the developing spinal cord led to strong defects in commissural axon trajectories at the midline intermediate target. These results reveal a highly distinctive axonal enrichment of IMP2, show that it interacts with a network of axon guidance-related mRNAs, and reveal that it is required for normal axon pathfinding during vertebrate development.
KW - Axon guidance
KW - Igf2bp2
KW - IMP2
KW - RNA interactome
KW - RNA-binding protein
U2 - 10.1242/dev.128348
DO - 10.1242/dev.128348
M3 - Journal article
C2 - 27385015
AN - SCOPUS:84982952422
VL - 143
SP - 2753
EP - 2759
JO - Development
JF - Development
SN - 0950-1991
IS - 15
ER -
ID: 179126718