Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis

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Standard

Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis. / von Essen, Marina Rode; Ammitzboll, Cecilie; Bornsen, Lars; Sellebjerg, Finn.

I: Clinical Immunology, Bind 230, 108817, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

von Essen, MR, Ammitzboll, C, Bornsen, L & Sellebjerg, F 2021, 'Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis', Clinical Immunology, bind 230, 108817. https://doi.org/10.1016/j.clim.2021.108817

APA

von Essen, M. R., Ammitzboll, C., Bornsen, L., & Sellebjerg, F. (2021). Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis. Clinical Immunology, 230, [108817]. https://doi.org/10.1016/j.clim.2021.108817

Vancouver

von Essen MR, Ammitzboll C, Bornsen L, Sellebjerg F. Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis. Clinical Immunology. 2021;230. 108817. https://doi.org/10.1016/j.clim.2021.108817

Author

von Essen, Marina Rode ; Ammitzboll, Cecilie ; Bornsen, Lars ; Sellebjerg, Finn. / Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis. I: Clinical Immunology. 2021 ; Bind 230.

Bibtex

@article{d5891291751d419d97a260561a3ba626,
title = "Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis",
abstract = "Many studies have analyzed myelin-reactivity of T cells in multiple sclerosis (MS); however, with conflicting results. In this study we compare methods to determine myelin reactivity of T cells and aim to delineate the cause of inconsistency in the literature. Challenging T cells with myelin antigens we found a significant increase in antigen-reactivity of T cells from patients with MS using an ELISpot-assay, in contrast to a CFSE-dilution assay. Comparing the two assays showed that the myelin-reactive T cells detected in the ELISpot-assay originated primarily from effector memory T cells in contrast to the myelin-reactive T cells of the CFSE-assay representing a population of both naive, central memory and effector memory T cells. This diversity in T cell populations activated in the two assays likely contribute to the discrepancy found in the literature and encourages thorough considerations when choosing an assay to determine antigen-specificity of T cells in future studies.",
keywords = "Myelin-reactive T cells, Assessing current methods, Multiple sclerosis, BASIC-PROTEIN, OLIGODENDROCYTE GLYCOPROTEIN, PROTEOLIPID PROTEIN, CEREBROSPINAL-FLUID, CYTOKINE PROFILE, TARGET ANTIGENS, FREQUENCY, RESPONSES, PEPTIDES, BLOOD",
author = "{von Essen}, {Marina Rode} and Cecilie Ammitzboll and Lars Bornsen and Finn Sellebjerg",
year = "2021",
doi = "10.1016/j.clim.2021.108817",
language = "English",
volume = "230",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Assessment of commonly used methods to determine myelin-reactivity of T cells in multiple sclerosis

AU - von Essen, Marina Rode

AU - Ammitzboll, Cecilie

AU - Bornsen, Lars

AU - Sellebjerg, Finn

PY - 2021

Y1 - 2021

N2 - Many studies have analyzed myelin-reactivity of T cells in multiple sclerosis (MS); however, with conflicting results. In this study we compare methods to determine myelin reactivity of T cells and aim to delineate the cause of inconsistency in the literature. Challenging T cells with myelin antigens we found a significant increase in antigen-reactivity of T cells from patients with MS using an ELISpot-assay, in contrast to a CFSE-dilution assay. Comparing the two assays showed that the myelin-reactive T cells detected in the ELISpot-assay originated primarily from effector memory T cells in contrast to the myelin-reactive T cells of the CFSE-assay representing a population of both naive, central memory and effector memory T cells. This diversity in T cell populations activated in the two assays likely contribute to the discrepancy found in the literature and encourages thorough considerations when choosing an assay to determine antigen-specificity of T cells in future studies.

AB - Many studies have analyzed myelin-reactivity of T cells in multiple sclerosis (MS); however, with conflicting results. In this study we compare methods to determine myelin reactivity of T cells and aim to delineate the cause of inconsistency in the literature. Challenging T cells with myelin antigens we found a significant increase in antigen-reactivity of T cells from patients with MS using an ELISpot-assay, in contrast to a CFSE-dilution assay. Comparing the two assays showed that the myelin-reactive T cells detected in the ELISpot-assay originated primarily from effector memory T cells in contrast to the myelin-reactive T cells of the CFSE-assay representing a population of both naive, central memory and effector memory T cells. This diversity in T cell populations activated in the two assays likely contribute to the discrepancy found in the literature and encourages thorough considerations when choosing an assay to determine antigen-specificity of T cells in future studies.

KW - Myelin-reactive T cells

KW - Assessing current methods

KW - Multiple sclerosis

KW - BASIC-PROTEIN

KW - OLIGODENDROCYTE GLYCOPROTEIN

KW - PROTEOLIPID PROTEIN

KW - CEREBROSPINAL-FLUID

KW - CYTOKINE PROFILE

KW - TARGET ANTIGENS

KW - FREQUENCY

KW - RESPONSES

KW - PEPTIDES

KW - BLOOD

U2 - 10.1016/j.clim.2021.108817

DO - 10.1016/j.clim.2021.108817

M3 - Journal article

C2 - 34352391

VL - 230

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

M1 - 108817

ER -

ID: 279624327