Alterations in blood microbiota after colonic cancer surgery
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Alterations in blood microbiota after colonic cancer surgery. / Søby, J. H.; Watt, S. K.; Vogelsang, R. P.; Servant, F.; Lelouvier, B.; Raskov, H.; Knop, F. K.; Gogenur, I.
In: BJS open, Vol. 4, No. 6, 2020, p. 1227-1237.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Alterations in blood microbiota after colonic cancer surgery
AU - Søby, J. H.
AU - Watt, S. K.
AU - Vogelsang, R. P.
AU - Servant, F.
AU - Lelouvier, B.
AU - Raskov, H.
AU - Knop, F. K.
AU - Gogenur, I.
PY - 2020
Y1 - 2020
N2 - Background: Mechanisms contributing to the perioperative stress response remain poorly understood. This study investigated changes in the amount of bacterial DNA in blood and the diversity of blood microbiota in the perioperative period in patients undergoing minimally invasive surgery for colonic cancer in an enhanced recovery after surgery setting.Methods: DNA encoding the bacterial 16S ribosomal RNA gene (16S rDNA) in whole blood obtained the day before surgery, and on postoperative day (POD) 1 and POD 10-14 was amplified and quantified by PCR before sequencing for taxonomic assignment. Richness, evenness and similarity measures were calculated to compare microbiota between days. Differences in relative abundance were analysed using the linear discriminant analysis effect size (LEfSe) algorithm.Results: Thirty patients were included between January and July 2016. The concentration of bacterial 16S rDNA in blood increased between the day before surgery and POD 1 (P = 0.025). Bacterial richness was lower on POD 10-14 than on the day before surgery and POD 1 (bothP <0 center dot 001). LEfSe analysis comparing the day before surgery and POD 10-14 identified changes in the abundance of several bacteria, includingFusobacterium nucleatum, which was relatively enriched on POD 10-14.Conclusion: These findings suggest that the blood of patients with colonic cancer harbours bacterial 16S rDNA, which increases in concentration after surgery.
AB - Background: Mechanisms contributing to the perioperative stress response remain poorly understood. This study investigated changes in the amount of bacterial DNA in blood and the diversity of blood microbiota in the perioperative period in patients undergoing minimally invasive surgery for colonic cancer in an enhanced recovery after surgery setting.Methods: DNA encoding the bacterial 16S ribosomal RNA gene (16S rDNA) in whole blood obtained the day before surgery, and on postoperative day (POD) 1 and POD 10-14 was amplified and quantified by PCR before sequencing for taxonomic assignment. Richness, evenness and similarity measures were calculated to compare microbiota between days. Differences in relative abundance were analysed using the linear discriminant analysis effect size (LEfSe) algorithm.Results: Thirty patients were included between January and July 2016. The concentration of bacterial 16S rDNA in blood increased between the day before surgery and POD 1 (P = 0.025). Bacterial richness was lower on POD 10-14 than on the day before surgery and POD 1 (bothP <0 center dot 001). LEfSe analysis comparing the day before surgery and POD 10-14 identified changes in the abundance of several bacteria, includingFusobacterium nucleatum, which was relatively enriched on POD 10-14.Conclusion: These findings suggest that the blood of patients with colonic cancer harbours bacterial 16S rDNA, which increases in concentration after surgery.
KW - COMPLETE MESOCOLIC EXCISION
KW - BACTERIAL TRANSLOCATION
KW - INFLAMMATION
KW - GUIDELINES
KW - SURVIVAL
KW - TRAUMA
KW - CELLS
U2 - 10.1002/bjs5.50357
DO - 10.1002/bjs5.50357
M3 - Journal article
C2 - 33022149
VL - 4
SP - 1227
EP - 1237
JO - BJS open
JF - BJS open
SN - 2474-9842
IS - 6
ER -
ID: 250213751