Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets. / Kaur, Simranjeet; Mirza, Aashiq H; Pociot, Flemming.
In: Non-coding RNA, Vol. 4, No. 4, 38, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Cell Type-Selective Expression of Circular RNAs in Human Pancreatic Islets
AU - Kaur, Simranjeet
AU - Mirza, Aashiq H
AU - Pociot, Flemming
PY - 2018
Y1 - 2018
N2 - Understanding distinct cell-type specific gene expression in human pancreatic islets is important for developing islet regeneration strategies and therapies to improve β-cell function in type 1 diabetes (T1D). While numerous transcriptome-wide studies on human islet cell-types have focused on protein-coding genes, the non-coding repertoire, such as long non-coding RNA, including circular RNAs, remains mostly unexplored. Here, we explored transcriptional landscape of human α-, β-, and exocrine cells from published total RNA sequencing (RNA-seq) datasets to identify circular RNAs (circRNAs). Our analysis revealed that circRNAs are highly abundant in both α- and β-cells. We identified 10,830 high-confidence circRNAs expressed in human α-, β-, and exocrine cells. The most highly expressed candidates were MAN1A2, RMST, and HIPK3 across the three cell-types. Alternate circular isoforms were observed for circRNAs in the three cell-types, indicative of potential distinct functions. Highly selective α- and β-cell circRNAs were identified, which is suggestive of their potential role in regulating β-cell function.
AB - Understanding distinct cell-type specific gene expression in human pancreatic islets is important for developing islet regeneration strategies and therapies to improve β-cell function in type 1 diabetes (T1D). While numerous transcriptome-wide studies on human islet cell-types have focused on protein-coding genes, the non-coding repertoire, such as long non-coding RNA, including circular RNAs, remains mostly unexplored. Here, we explored transcriptional landscape of human α-, β-, and exocrine cells from published total RNA sequencing (RNA-seq) datasets to identify circular RNAs (circRNAs). Our analysis revealed that circRNAs are highly abundant in both α- and β-cells. We identified 10,830 high-confidence circRNAs expressed in human α-, β-, and exocrine cells. The most highly expressed candidates were MAN1A2, RMST, and HIPK3 across the three cell-types. Alternate circular isoforms were observed for circRNAs in the three cell-types, indicative of potential distinct functions. Highly selective α- and β-cell circRNAs were identified, which is suggestive of their potential role in regulating β-cell function.
U2 - 10.3390/ncrna4040038
DO - 10.3390/ncrna4040038
M3 - Journal article
C2 - 30486482
VL - 4
JO - Non-coding RNA
JF - Non-coding RNA
SN - 2311-553X
IS - 4
M1 - 38
ER -
ID: 216519859