Expression of ICAM-1 in colon epithelial cells: an ultrastructural study performed on in vivo and in vitro models
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Expression of ICAM-1 in colon epithelial cells : an ultrastructural study performed on in vivo and in vitro models. / Vainer, Ben; Sørensen, Susanne; Seidelin, Jakob; Nielsen, Ole Haagen; Horn, Thomas.
In: Virchows Archiv, Vol. 443, No. 6, 12.2003, p. 774-81.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Expression of ICAM-1 in colon epithelial cells
T2 - an ultrastructural study performed on in vivo and in vitro models
AU - Vainer, Ben
AU - Sørensen, Susanne
AU - Seidelin, Jakob
AU - Nielsen, Ole Haagen
AU - Horn, Thomas
PY - 2003/12
Y1 - 2003/12
N2 - BACKGROUND: Studies have suggested that in ulcerative colitis (UC), intercellular adhesion molecule-1 (ICAM-1) is involved in migration of leukocytes toward the colonic epithelium. A suitable in vitro model of chronic colonic inflammation does not exist, and the role of the epithelium is based on monolayers of cancer cells. Conflicting results exist on epithelial ICAM-1 expression, and the aim of this study was to compare the expression in various models of colonic epithelium.MATERIALS AND METHODS: Colonic biopsies from four UC patients and four controls were examined by cryoimmuno-electron microscopy using ICAM-1-antibodies. In four other controls, the epithelium was isolated from colonic biopsies, embedded in collagen, and evaluated similarly. Isolated crypts and cultured cancer cells were stimulated with interferon-gamma (IFN-gamma) or tumor necrosis factor-alpha (TNF-alpha).RESULTS: ICAM-1 was not expressed in the biopsies. In contrast, HT29 cells and the collagen-embedded crypts expressed ICAM-1 on the apical membranes proximal to the junctional complexes when stimulated with IFN-gamma or TNF-alpha in a dose-related manner.CONCLUSIONS: ICAM-1 is not expressed on colonic epithelium in vivo. However, both colonocytes and HT29 cells were capable of expressing ICAM-1 on their apical membranes in response to supraphysiologic cytokine concentrations. These observations question the justification of extrapolating observations from colon cancer cell lines to in vivo inflammatory conditions.
AB - BACKGROUND: Studies have suggested that in ulcerative colitis (UC), intercellular adhesion molecule-1 (ICAM-1) is involved in migration of leukocytes toward the colonic epithelium. A suitable in vitro model of chronic colonic inflammation does not exist, and the role of the epithelium is based on monolayers of cancer cells. Conflicting results exist on epithelial ICAM-1 expression, and the aim of this study was to compare the expression in various models of colonic epithelium.MATERIALS AND METHODS: Colonic biopsies from four UC patients and four controls were examined by cryoimmuno-electron microscopy using ICAM-1-antibodies. In four other controls, the epithelium was isolated from colonic biopsies, embedded in collagen, and evaluated similarly. Isolated crypts and cultured cancer cells were stimulated with interferon-gamma (IFN-gamma) or tumor necrosis factor-alpha (TNF-alpha).RESULTS: ICAM-1 was not expressed in the biopsies. In contrast, HT29 cells and the collagen-embedded crypts expressed ICAM-1 on the apical membranes proximal to the junctional complexes when stimulated with IFN-gamma or TNF-alpha in a dose-related manner.CONCLUSIONS: ICAM-1 is not expressed on colonic epithelium in vivo. However, both colonocytes and HT29 cells were capable of expressing ICAM-1 on their apical membranes in response to supraphysiologic cytokine concentrations. These observations question the justification of extrapolating observations from colon cancer cell lines to in vivo inflammatory conditions.
KW - Adult
KW - Colitis, Ulcerative
KW - Colon
KW - Colonic Neoplasms
KW - Female
KW - HT29 Cells
KW - Humans
KW - Intercellular Adhesion Molecule-1
KW - Interferon-gamma
KW - Intestinal Mucosa
KW - Male
KW - Microscopy, Electron
KW - Tumor Necrosis Factor-alpha
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1007/s00428-003-0900-5
DO - 10.1007/s00428-003-0900-5
M3 - Journal article
C2 - 14564519
VL - 443
SP - 774
EP - 781
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 6
ER -
ID: 173051858