Elevated polygenic burden for autism is associated with differential DNA methylation at birth
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Elevated polygenic burden for autism is associated with differential DNA methylation at birth. / Hannon, Eilis; Schendel, Diana; Ladd-Acosta, Christine; Grove, Jakob; Hansen, Christine Soholm; Andrews, Shan V.; Hougaard, David Michael; Bresnahan, Michaeline; Mors, Ole; Hollegaard, Mads Vilhelm; Bækvad-Hansen, Marie; Hornig, Mady; Mortensen, Preben Bo; Børglum, Anders D.; Werge, Thomas; Pedersen, Marianne Giørtz; Nordentoft, Merete; Buxbaum, Joseph; Fallin, M. Daniele; Bybjerg-Grauholm, Jonas; Reichenberg, Abraham; Mill, Jonathan.
In: Genome Medicine, Vol. 10, 19, 28.03.2018.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Elevated polygenic burden for autism is associated with differential DNA methylation at birth
AU - Hannon, Eilis
AU - Schendel, Diana
AU - Ladd-Acosta, Christine
AU - Grove, Jakob
AU - Hansen, Christine Soholm
AU - Andrews, Shan V.
AU - Hougaard, David Michael
AU - Bresnahan, Michaeline
AU - Mors, Ole
AU - Hollegaard, Mads Vilhelm
AU - Bækvad-Hansen, Marie
AU - Hornig, Mady
AU - Mortensen, Preben Bo
AU - Børglum, Anders D.
AU - Werge, Thomas
AU - Pedersen, Marianne Giørtz
AU - Nordentoft, Merete
AU - Buxbaum, Joseph
AU - Fallin, M. Daniele
AU - Bybjerg-Grauholm, Jonas
AU - Reichenberg, Abraham
AU - Mill, Jonathan
PY - 2018/3/28
Y1 - 2018/3/28
N2 - BackgroundAutism spectrum disorder (ASD) is a severe neurodevelopmental disorder characterized by deficits in social communication and restricted, repetitive behaviors, interests, or activities. The etiology of ASD involves both inherited and environmental risk factors, with epigenetic processes hypothesized as one mechanism by which both genetic and non-genetic variation influence gene regulation and pathogenesis. The aim of this study was to identify DNA methylation biomarkers of ASD detectable at birth.MethodsWe quantified neonatal methylomic variation in 1263 infants—of whom ~ 50% went on to subsequently develop ASD—using DNA isolated from archived blood spots taken shortly after birth. We used matched genotype data from the same individuals to examine the molecular consequences of ASD-associated genetic risk variants, identifying methylomic variation associated with elevated polygenic burden for ASD. In addition, we performed DNA methylation quantitative trait loci (mQTL) mapping to prioritize target genes from ASD GWAS findings.ResultsWe identified robust epigenetic signatures of gestational age and prenatal tobacco exposure, confirming the utility of DNA methylation data generated from neonatal blood spots. Although we did not identify specific loci showing robust differences in neonatal DNA methylation associated with later ASD, there was a significant association between increased polygenic burden for autism and methylomic variation at specific loci. Each unit of elevated ASD polygenic risk score was associated with a mean increase in DNA methylation of − 0.14% at two CpG sites located proximal to a robust GWAS signal for ASD on chromosome 8.ConclusionsThis study is the largest analysis of DNA methylation in ASD undertaken and the first to integrate genetic and epigenetic variation at birth. We demonstrate the utility of using a polygenic risk score to identify molecular variation associated with disease, and of using mQTL to refine the functional and regulatory variation associated with ASD risk variants.
AB - BackgroundAutism spectrum disorder (ASD) is a severe neurodevelopmental disorder characterized by deficits in social communication and restricted, repetitive behaviors, interests, or activities. The etiology of ASD involves both inherited and environmental risk factors, with epigenetic processes hypothesized as one mechanism by which both genetic and non-genetic variation influence gene regulation and pathogenesis. The aim of this study was to identify DNA methylation biomarkers of ASD detectable at birth.MethodsWe quantified neonatal methylomic variation in 1263 infants—of whom ~ 50% went on to subsequently develop ASD—using DNA isolated from archived blood spots taken shortly after birth. We used matched genotype data from the same individuals to examine the molecular consequences of ASD-associated genetic risk variants, identifying methylomic variation associated with elevated polygenic burden for ASD. In addition, we performed DNA methylation quantitative trait loci (mQTL) mapping to prioritize target genes from ASD GWAS findings.ResultsWe identified robust epigenetic signatures of gestational age and prenatal tobacco exposure, confirming the utility of DNA methylation data generated from neonatal blood spots. Although we did not identify specific loci showing robust differences in neonatal DNA methylation associated with later ASD, there was a significant association between increased polygenic burden for autism and methylomic variation at specific loci. Each unit of elevated ASD polygenic risk score was associated with a mean increase in DNA methylation of − 0.14% at two CpG sites located proximal to a robust GWAS signal for ASD on chromosome 8.ConclusionsThis study is the largest analysis of DNA methylation in ASD undertaken and the first to integrate genetic and epigenetic variation at birth. We demonstrate the utility of using a polygenic risk score to identify molecular variation associated with disease, and of using mQTL to refine the functional and regulatory variation associated with ASD risk variants.
KW - Autism
KW - DNA methylation
KW - Genetics
KW - Neonatal
KW - Genome-wide association study (GWAS)
KW - Epigenome-wide association study (EWAS)
KW - Birth
KW - DNA methylation quantitative trait loci (mQTL)
KW - Polygenic risk score
KW - Prenatal smoking
U2 - 10.1186/s13073-018-0527-4
DO - 10.1186/s13073-018-0527-4
M3 - Journal article
C2 - 29587883
VL - 10
JO - Genome Medicine
JF - Genome Medicine
SN - 1756-994X
M1 - 19
ER -
ID: 209290511