A sensitive and reproducible method for the determination of subnanogram quantities of immunoglobulin E (IgE)

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An immunoradiometric assay for immunoglobulin E (IgE) using high adsorption polystyrene test tubes as the solid phase (the 'Maxisorp' assay) is described. The sensitivity of the method was found to be 50 pg IgE/ml and the within-assay reproducibility was 3-7%. The accuracy was estimated by means of a comparison between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude that the Maxisorp assay is a fast and reliable assay for IgE determination in cord blood, cell culture supernatants and other highly diluted IgE preparations.

Original languageEnglish
JournalJournal of Immunological Methods
Volume92
Issue number1
Pages (from-to)131-6
Number of pages6
ISSN0022-1759
Publication statusPublished - 21 Aug 1986

    Research areas

  • Antibody Specificity, Binding, Competitive, Cross Reactions, Dose-Response Relationship, Immunologic, Humans, Immunoglobulin A, Immunoglobulin E, Immunoglobulin G, Immunoglobulin M, Immunosorbent Techniques, Radioimmunoassay, Journal Article, Research Support, Non-U.S. Gov't

ID: 169714843