Assessment of P-glycoprotein-dependent drug transport in isolated rat hepatocytes using rhodamine 123
Research output: Contribution to journal › Journal article › Research › peer-review
To test the activity of P-170 glycoprotein in isolated hepatocytes, a method has been developed employing the fluorescent dye rhodamine 123 (R-123). The uptake of R-123 by both freshly isolated and 4-hr-plated hepatocytes depends on dye concentration, time of incubation, and cell number. The efflux of R-123 from cells is inhibited by sodium azide and by verapamil. In standard conditions the efficiency of efflux of R-123 from cells correlates with the relative amount of immunoblottable glycoprotein. The method has been applied to detection of P-170 activity in hepatocytes from animals of different ages as well as from carcinogen-treated animals. The proposed assay appears a simple and adequate tool for the functional assessment of multidrug transporter in liver.
Original language | English |
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Journal | Cell Biology and Toxicology |
Volume | 9 |
Issue number | 3 |
Pages (from-to) | 235-41 |
Number of pages | 7 |
ISSN | 0742-2091 |
DOIs | |
Publication status | Published - 1 Jul 1993 |
Externally published | Yes |
- ATP Binding Cassette Transporter, Subfamily B, Member 1, Animals, Azides/pharmacology, Biological Transport, Active/drug effects, Carrier Proteins/metabolism, Cell Membrane/drug effects, Drug Resistance, Fluorescent Dyes/pharmacokinetics, In Vitro Techniques, Liver/drug effects, Male, Membrane Glycoproteins/metabolism, Rats, Rats, Sprague-Dawley, Rhodamine 123, Rhodamines/pharmacokinetics, Sodium Azide, Verapamil/pharmacology
Research areas
ID: 257734003