Circulating AMH reflects ovarian morphology by magnetic resonance imaging and 3D ultrasound in 121 healthy girls
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Circulating AMH reflects ovarian morphology by magnetic resonance imaging and 3D ultrasound in 121 healthy girls. / Hagen, Casper P; Mouritsen, Annette; Mieritz, Mikkel G; Tinggaard, Jeanette; Wohlfart-Veje, Christine; Fallentin, Eva; Brocks, Vibeke; Sundberg, Karin; Jensen, Lisa Neerup; Anderson, Richard A; Juul, Anders; Main, Katharina Maria.
In: Journal of Clinical Endocrinology and Metabolism, Vol. 100, No. 3, 03.2015, p. 880-90.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Circulating AMH reflects ovarian morphology by magnetic resonance imaging and 3D ultrasound in 121 healthy girls
AU - Hagen, Casper P
AU - Mouritsen, Annette
AU - Mieritz, Mikkel G
AU - Tinggaard, Jeanette
AU - Wohlfart-Veje, Christine
AU - Fallentin, Eva
AU - Brocks, Vibeke
AU - Sundberg, Karin
AU - Jensen, Lisa Neerup
AU - Anderson, Richard A
AU - Juul, Anders
AU - Main, Katharina Maria
PY - 2015/3
Y1 - 2015/3
N2 - CONTEXT: In adult women, Anti-Müllerian hormone (AMH) is produced by small growing follicles, and circulating levels of AMH reflect the number of antral follicles as well as primordial follicles. Whether AMH reflects follicle numbers in healthy girls remains to be elucidated.OBJECTIVE: This study aimed to evaluate whether serum levels of AMH reflects ovarian morphology in healthy girls.DESIGN AND SETTING: This was a population-based cohort study involving the general community.PARTICIPANTS: Included in the study were 121 healthy girls 9.8-14.7 years of age.MAIN OUTCOME MEASURES: Clinical examination, including pubertal breast stage (Tanner's classification B1-5), ovarian volume, as well as the number and size of antral follicles were assessed by two independent modalities: magnetic resonance imaging (MRI), Ellipsoid volume, follicles ≥2 mm; and Transabdominal ultrasound, Ellipsoid and 3D volume, follicles ≥1 mm. Circulating levels of AMH, inhibin B, estradiol, FSH, and LH were assessed by immunoassays; T and androstenedione were assessed by liquid chromatography-tandem mass spectrometry.RESULTS: AMH reflected the number of small (MRI 2-3 mm) and medium (4-6 mm) follicles (Pearson's Rho [r] = 0.531 and r = 0.512, P < .001) but not large follicles (≥7 mm) (r = 0.109, P = .323). In multiple regression analysis, small and medium follicles (MRI ≤ 6 mm) remained the main contributors to circulating AMH (β, 0.501; P < .001) whereas the correlation between AMH and estradiol was negative (β, -0.318; P = .005). In early puberty (B1-B3), the number of AMH-producing follicles (2-6 mm) correlated positively with pubertal stages (r = 0.453, P = .001), whereas AMH levels were unaffected (-0.183, P = .118).CONCLUSIONS: Similarly to adult women, small and medium antral follicles (≤6 mm) were the main contributors to circulating levels of AMH in girls.
AB - CONTEXT: In adult women, Anti-Müllerian hormone (AMH) is produced by small growing follicles, and circulating levels of AMH reflect the number of antral follicles as well as primordial follicles. Whether AMH reflects follicle numbers in healthy girls remains to be elucidated.OBJECTIVE: This study aimed to evaluate whether serum levels of AMH reflects ovarian morphology in healthy girls.DESIGN AND SETTING: This was a population-based cohort study involving the general community.PARTICIPANTS: Included in the study were 121 healthy girls 9.8-14.7 years of age.MAIN OUTCOME MEASURES: Clinical examination, including pubertal breast stage (Tanner's classification B1-5), ovarian volume, as well as the number and size of antral follicles were assessed by two independent modalities: magnetic resonance imaging (MRI), Ellipsoid volume, follicles ≥2 mm; and Transabdominal ultrasound, Ellipsoid and 3D volume, follicles ≥1 mm. Circulating levels of AMH, inhibin B, estradiol, FSH, and LH were assessed by immunoassays; T and androstenedione were assessed by liquid chromatography-tandem mass spectrometry.RESULTS: AMH reflected the number of small (MRI 2-3 mm) and medium (4-6 mm) follicles (Pearson's Rho [r] = 0.531 and r = 0.512, P < .001) but not large follicles (≥7 mm) (r = 0.109, P = .323). In multiple regression analysis, small and medium follicles (MRI ≤ 6 mm) remained the main contributors to circulating AMH (β, 0.501; P < .001) whereas the correlation between AMH and estradiol was negative (β, -0.318; P = .005). In early puberty (B1-B3), the number of AMH-producing follicles (2-6 mm) correlated positively with pubertal stages (r = 0.453, P = .001), whereas AMH levels were unaffected (-0.183, P = .118).CONCLUSIONS: Similarly to adult women, small and medium antral follicles (≤6 mm) were the main contributors to circulating levels of AMH in girls.
KW - Adolescent
KW - Anti-Mullerian Hormone
KW - Child
KW - Cohort Studies
KW - Female
KW - Health
KW - Humans
KW - Imaging, Three-Dimensional
KW - Magnetic Resonance Imaging
KW - Organ Size
KW - Ovarian Follicle
KW - Ovary
KW - Ultrasonography
U2 - 10.1210/jc.2014-3336
DO - 10.1210/jc.2014-3336
M3 - Journal article
C2 - 25485726
VL - 100
SP - 880
EP - 890
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
SN - 0021-972X
IS - 3
ER -
ID: 162026708