Hb A2-Calderdale [δ2(NA2)His→Asn; HBD: c.7C>A] and Misdiagnosis of Type 2 Diabetes Mellitus Due to Interference with Hb A1c When Using Cation Exchange High Performance Liquid Chromatography: A Case Report
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The Hb A2-Calderdale variant [δ2(NA2)His→Asn, HBD: c.7C>A] was described as a novel variant in 2014. However, a high performance liquid chromatography (HPLC) peak was never identified indicating that this fraction could be ‘hiding’ somewhere else in the chromatogram. In this case report, we present evidence that the physiochemical properties of Hb A2-Calderdale resemble those of Hb A1c, resulting in a coelution in variant mode on the Tosoh G8 but not the Tosoh G11. This coelution results in an overestimation of Hb A1c and can potentially cause misdiagnosis of type 2 diabetes mellitus (T2DM).
| Original language | English |
|---|---|
| Journal | Hemoglobin |
| Volume | 45 |
| Issue number | 3 |
| Pages (from-to) | 207-209 |
| ISSN | 0363-0269 |
| DOIs | |
| Publication status | Published - 2021 |
Bibliographical note
Funding Information:
The authors thank Anette Glibstrup (Medical Laboratory Technologist, Rigshospitalet, Copenhagen, Denmark) and Charlotte Amtoft (Product Specialist, Sysmex Nordic Aps, Copenhagen, Denmark) for excellent technical assistance, and Stefan Stender, PhD, MD (Bispebjerg and Frederiksberg Hospital, Copenhagen, Denmark), for helpful comments on an earlier version of this short communication.
Publisher Copyright:
© 2021 Informa UK Limited, trading as Taylor & Francis Group.
- Hb A, Hb A-Calderdale, high performance liquid chromatography (HPLC), δ-Globin gene
Research areas
ID: 302070553