The use of core genome multilocus sequence typing to determine the duration of vancomycin-resistant Enterococcus faecium outbreaks
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The use of core genome multilocus sequence typing to determine the duration of vancomycin-resistant Enterococcus faecium outbreaks. / Søndergaard Knudsen, Maja; Maria Cecilia Rubin, Ingrid; Gisselø, Katrine; Mollerup, Sarah; Munk Petersen, Andreas; Pinholt, Mette; Westh, Henrik; Damkjaer Bartels, Mette.
In: A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica (Online), Vol. 130, No. 6, 03.2022, p. 323-329.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - The use of core genome multilocus sequence typing to determine the duration of vancomycin-resistant Enterococcus faecium outbreaks
AU - Søndergaard Knudsen, Maja
AU - Maria Cecilia Rubin, Ingrid
AU - Gisselø, Katrine
AU - Mollerup, Sarah
AU - Munk Petersen, Andreas
AU - Pinholt, Mette
AU - Westh, Henrik
AU - Damkjaer Bartels, Mette
N1 - This article is protected by copyright. All rights reserved.
PY - 2022/3
Y1 - 2022/3
N2 - BACKGROUND: The prevalence of vancomycin-resistant Enterococcus faecium has increased rapidly, and in Denmark we are facing an endemic outbreak situation in hospitals.OBJECTIVES: The aim of this study was to use whole genome sequencing (WGS) and core genome multilocus sequencing typing (cgMLST) to determine the duration of VREfm outbreaks and thereby evaluate the effect of our infection control strategies.METHODS: We included all VREfm isolates from six hospitals in the Capital Region of Denmark that were sequenced between 2012 and 2020. Ward data was collected from our laboratory information system. A ward outbreak was defined as two patient samples from the same ward within a period of 30 days belonging to the same cgMLST cluster. cgMLST complex types were determined using Ridom SeqSphere v7.2.3, where a maximum of 20 allelic differences between isolates defines a cluster.RESULTS: We included 1690 patient isolates between 2012 and 2020. Our collection consisted of 45 unique clusters and 227 ward outbreaks. The median duration of outbreaks was 20 days.CONCLUSION: We reported a median outbreak duration of VREfm outbreaks based on WGS data to be 20 days, and thus concluded that our infection control precautions are adequate.
AB - BACKGROUND: The prevalence of vancomycin-resistant Enterococcus faecium has increased rapidly, and in Denmark we are facing an endemic outbreak situation in hospitals.OBJECTIVES: The aim of this study was to use whole genome sequencing (WGS) and core genome multilocus sequencing typing (cgMLST) to determine the duration of VREfm outbreaks and thereby evaluate the effect of our infection control strategies.METHODS: We included all VREfm isolates from six hospitals in the Capital Region of Denmark that were sequenced between 2012 and 2020. Ward data was collected from our laboratory information system. A ward outbreak was defined as two patient samples from the same ward within a period of 30 days belonging to the same cgMLST cluster. cgMLST complex types were determined using Ridom SeqSphere v7.2.3, where a maximum of 20 allelic differences between isolates defines a cluster.RESULTS: We included 1690 patient isolates between 2012 and 2020. Our collection consisted of 45 unique clusters and 227 ward outbreaks. The median duration of outbreaks was 20 days.CONCLUSION: We reported a median outbreak duration of VREfm outbreaks based on WGS data to be 20 days, and thus concluded that our infection control precautions are adequate.
U2 - 10.1111/apm.13216
DO - 10.1111/apm.13216
M3 - Review
C2 - 35253272
VL - 130
SP - 323
EP - 329
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 6
ER -
ID: 300671616