TY - JOUR

T1 - Validation of a novel automated system, Fluispotter®, for serial sampling of dried blood spots

AU - Krogh, Jesper

AU - Plomgaard, Peter

AU - Frikke-Schmidt, Ruth

AU - Velschow, Sten

AU - Johannesen, Jesper

AU - Hilsted, Linda Maria

AU - Schrøder, Malene

AU - Feldt-Rasmussen, Ulla

N1 - Publisher Copyright: © 2023 the author(s) Published by Bioscientifica Ltd.

PY - 2023

Y1 - 2023

N2 - Repeated blood sampling is required in certain clinical and research settings, which is currently performed by drawing blood from venous catheters requiring manual handling of each sample at the time of collection. A novel body-worn device for repeated serial samples, Fluispotter®, with automated extraction, collection, and storage of up to 20 venous dried blood spot samples over the course of 20 h may overcome problems with current methods for serial sampling. The purpose of this study was to assess the performance and safety of Fluispotter for the first time in healthy subjects. Fluispotter consists of a cartridge with tubinga reservoir for flushing solution, pumps and filter paper, and a multi-lumen catheter placed in the brachial vein. We recruited healthy subjects for testing in an in-hospital setting. Fluispottewas attached by an anesthesiologist to 22 healthy subjects of which 9/22 (40.9%) participants had all 20 samples taken, which was lower than the goal of complete sampling in 80% of the subjects (P = 0.02). The main reason for sample failure was clogging of blood flow which was observed in 11/22 (50%) of the participants. No serious adverse events occurred, and the participants rated the pain from the insertion and the removal of catheter as very low. A cortisol profile showed nadir values at midnight and highest values at 05:00 h. Although full sampling was not successful in all participants, the Fluispotter technology proved safe and highly acceptable to the participants producing the expected cortisol profile without the requirement of staff during sample collection.

AB - Repeated blood sampling is required in certain clinical and research settings, which is currently performed by drawing blood from venous catheters requiring manual handling of each sample at the time of collection. A novel body-worn device for repeated serial samples, Fluispotter®, with automated extraction, collection, and storage of up to 20 venous dried blood spot samples over the course of 20 h may overcome problems with current methods for serial sampling. The purpose of this study was to assess the performance and safety of Fluispotter for the first time in healthy subjects. Fluispotter consists of a cartridge with tubinga reservoir for flushing solution, pumps and filter paper, and a multi-lumen catheter placed in the brachial vein. We recruited healthy subjects for testing in an in-hospital setting. Fluispottewas attached by an anesthesiologist to 22 healthy subjects of which 9/22 (40.9%) participants had all 20 samples taken, which was lower than the goal of complete sampling in 80% of the subjects (P = 0.02). The main reason for sample failure was clogging of blood flow which was observed in 11/22 (50%) of the participants. No serious adverse events occurred, and the participants rated the pain from the insertion and the removal of catheter as very low. A cortisol profile showed nadir values at midnight and highest values at 05:00 h. Although full sampling was not successful in all participants, the Fluispotter technology proved safe and highly acceptable to the participants producing the expected cortisol profile without the requirement of staff during sample collection.

KW - automated sampling

KW - cortisol

KW - diurnal

KW - dried blood spots

U2 - 10.1530/EC-23-0087

DO - 10.1530/EC-23-0087

M3 - Journal article

C2 - 36939600

AN - SCOPUS:85162740174

VL - 12

JO - Endocrine Connections

JF - Endocrine Connections

SN - 2049-3614

IS - 7

M1 - e230087

ER -