Blood group genotyping of blood donors: validation of a highly accurate routine method
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Blood group genotyping of blood donors : validation of a highly accurate routine method. / Krog, Grethe Risum; Rieneck, Klaus; Clausen, Frederik Banch; Steffensen, Rudi; Dziegiel, Morten Hanefeld.
In: Transfusion, Vol. 59, No. 10, 2019, p. 3264-3274.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Blood group genotyping of blood donors
T2 - validation of a highly accurate routine method
AU - Krog, Grethe Risum
AU - Rieneck, Klaus
AU - Clausen, Frederik Banch
AU - Steffensen, Rudi
AU - Dziegiel, Morten Hanefeld
N1 - © 2019 AABB.
PY - 2019
Y1 - 2019
N2 - BACKGROUND: In the past century, blood group determination using serology has been the standard method. Now, molecular methods are gaining traction, which provide additional and easily accessible information. Here we designed and validated a high-throughput extended genotyping setup.STUDY DESIGN AND METHODS: We developed 35 competitive allele-specific polymerase chain reaction assays for genotyping of blood donors. Samples from 1034 Danish blood donors were genotyped, and 45,314 red blood cell antigens and 6148 platelet antigens were predicted. Predicted phenotypes were compared with 16,119 serologic phenotypes.RESULTS: We found 62 discrepancies of which 43 were due to serology. After exclusion of the discrepancies caused by serology, the accuracy of genotyping was 99.9%. Of 17 discrepancies caused by the genotype, three were incorrect antigen-negative predictions and could potentially, as the solitary analysis, have caused an adverse transfusion reaction.CONCLUSION: We have established a robust and highly accurate blood group genotyping system with a very high capacity for screening blood donors. The system represents a significant improvement over the former serotyping-only procedure. Almost all new technology in medicine incurs increased costs, but the presented efficient genotyping system is a rare example of a significant qualitative and quantitative technologic progress that is also more cost-efficient than previous technologies.
AB - BACKGROUND: In the past century, blood group determination using serology has been the standard method. Now, molecular methods are gaining traction, which provide additional and easily accessible information. Here we designed and validated a high-throughput extended genotyping setup.STUDY DESIGN AND METHODS: We developed 35 competitive allele-specific polymerase chain reaction assays for genotyping of blood donors. Samples from 1034 Danish blood donors were genotyped, and 45,314 red blood cell antigens and 6148 platelet antigens were predicted. Predicted phenotypes were compared with 16,119 serologic phenotypes.RESULTS: We found 62 discrepancies of which 43 were due to serology. After exclusion of the discrepancies caused by serology, the accuracy of genotyping was 99.9%. Of 17 discrepancies caused by the genotype, three were incorrect antigen-negative predictions and could potentially, as the solitary analysis, have caused an adverse transfusion reaction.CONCLUSION: We have established a robust and highly accurate blood group genotyping system with a very high capacity for screening blood donors. The system represents a significant improvement over the former serotyping-only procedure. Almost all new technology in medicine incurs increased costs, but the presented efficient genotyping system is a rare example of a significant qualitative and quantitative technologic progress that is also more cost-efficient than previous technologies.
U2 - 10.1111/trf.15474
DO - 10.1111/trf.15474
M3 - Journal article
C2 - 31415105
VL - 59
SP - 3264
EP - 3274
JO - Transfusion
JF - Transfusion
SN - 0041-1132
IS - 10
ER -
ID: 228407800