Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs

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Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs. / Cheng, Shih-Chin; Sprong, Tom; Joosten, Leo A B; van der Meer, Jos W M; Kullberg, Bart-Jan; Hube, Bernhard; Schejbel, Lone; Garred, Peter; van Deuren, Marcel; Netea, Mihai G.

In: Central-European Journal of Immunology, Vol. 42, No. 4, 2012, p. 993-1004.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cheng, S-C, Sprong, T, Joosten, LAB, van der Meer, JWM, Kullberg, B-J, Hube, B, Schejbel, L, Garred, P, van Deuren, M & Netea, MG 2012, 'Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs', Central-European Journal of Immunology, vol. 42, no. 4, pp. 993-1004. https://doi.org/10.1002/eji.201142057

APA

Cheng, S-C., Sprong, T., Joosten, L. A. B., van der Meer, J. W. M., Kullberg, B-J., Hube, B., Schejbel, L., Garred, P., van Deuren, M., & Netea, M. G. (2012). Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs. Central-European Journal of Immunology, 42(4), 993-1004. https://doi.org/10.1002/eji.201142057

Vancouver

Cheng S-C, Sprong T, Joosten LAB, van der Meer JWM, Kullberg B-J, Hube B et al. Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs. Central-European Journal of Immunology. 2012;42(4):993-1004. https://doi.org/10.1002/eji.201142057

Author

Cheng, Shih-Chin ; Sprong, Tom ; Joosten, Leo A B ; van der Meer, Jos W M ; Kullberg, Bart-Jan ; Hube, Bernhard ; Schejbel, Lone ; Garred, Peter ; van Deuren, Marcel ; Netea, Mihai G. / Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs. In: Central-European Journal of Immunology. 2012 ; Vol. 42, No. 4. pp. 993-1004.

Bibtex

@article{67147e2993c9414ca8f53ea227e5f98d,
title = "Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs",
abstract = "In experimental studies, the role of complement in antifungal host defense has been attributed to its opsonizing capability. In this study, we report that in humans an activated complement system mainly augments Candida albicans-induced host proinflammatory cytokine production via C5a-C5aR signaling, while phagocytosis and intracellular killing of Candida are not influenced. By blocking the C5a-C5aR signaling pathway, either with anti-C5a antagonist antibodies or with the C5aR antagonist W-54001, C. albicans-induced IL-6 and IL-1β levels were significantly reduced. Recombinant C5a augmented cytokine production. In addition, using serum from patients with various complement deficiencies, we demonstrated a crucial role of C5, but not C6 or the membrane attack complex, in C. albicans-induced IL-6 and IL-1β production in monocytes. These findings reveal a central role of anaphylatoxin C5a in augmenting host proinflammatory cytokine production upon contact with C. albicans, and define the role of the complement system in anti-Candida host defense in humans.",
author = "Shih-Chin Cheng and Tom Sprong and Joosten, {Leo A B} and {van der Meer}, {Jos W M} and Bart-Jan Kullberg and Bernhard Hube and Lone Schejbel and Peter Garred and {van Deuren}, Marcel and Netea, {Mihai G}",
note = "{\textcopyright} 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
year = "2012",
doi = "10.1002/eji.201142057",
language = "English",
volume = "42",
pages = "993--1004",
journal = "Central-European Journal of Immunology",
issn = "1426-3912",
publisher = "Termedia sp. z o.o.",
number = "4",

}

RIS

TY - JOUR

T1 - Complement plays a central role in Candida albicans-induced cytokine production by human PBMCs

AU - Cheng, Shih-Chin

AU - Sprong, Tom

AU - Joosten, Leo A B

AU - van der Meer, Jos W M

AU - Kullberg, Bart-Jan

AU - Hube, Bernhard

AU - Schejbel, Lone

AU - Garred, Peter

AU - van Deuren, Marcel

AU - Netea, Mihai G

N1 - © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PY - 2012

Y1 - 2012

N2 - In experimental studies, the role of complement in antifungal host defense has been attributed to its opsonizing capability. In this study, we report that in humans an activated complement system mainly augments Candida albicans-induced host proinflammatory cytokine production via C5a-C5aR signaling, while phagocytosis and intracellular killing of Candida are not influenced. By blocking the C5a-C5aR signaling pathway, either with anti-C5a antagonist antibodies or with the C5aR antagonist W-54001, C. albicans-induced IL-6 and IL-1β levels were significantly reduced. Recombinant C5a augmented cytokine production. In addition, using serum from patients with various complement deficiencies, we demonstrated a crucial role of C5, but not C6 or the membrane attack complex, in C. albicans-induced IL-6 and IL-1β production in monocytes. These findings reveal a central role of anaphylatoxin C5a in augmenting host proinflammatory cytokine production upon contact with C. albicans, and define the role of the complement system in anti-Candida host defense in humans.

AB - In experimental studies, the role of complement in antifungal host defense has been attributed to its opsonizing capability. In this study, we report that in humans an activated complement system mainly augments Candida albicans-induced host proinflammatory cytokine production via C5a-C5aR signaling, while phagocytosis and intracellular killing of Candida are not influenced. By blocking the C5a-C5aR signaling pathway, either with anti-C5a antagonist antibodies or with the C5aR antagonist W-54001, C. albicans-induced IL-6 and IL-1β levels were significantly reduced. Recombinant C5a augmented cytokine production. In addition, using serum from patients with various complement deficiencies, we demonstrated a crucial role of C5, but not C6 or the membrane attack complex, in C. albicans-induced IL-6 and IL-1β production in monocytes. These findings reveal a central role of anaphylatoxin C5a in augmenting host proinflammatory cytokine production upon contact with C. albicans, and define the role of the complement system in anti-Candida host defense in humans.

U2 - 10.1002/eji.201142057

DO - 10.1002/eji.201142057

M3 - Journal article

C2 - 22531923

VL - 42

SP - 993

EP - 1004

JO - Central-European Journal of Immunology

JF - Central-European Journal of Immunology

SN - 1426-3912

IS - 4

ER -

ID: 48448940