Development of a fiber-based microfluidic flow cytometry platform using viscoelastic fluids for polydisperse particle suspensions
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Development of a fiber-based microfluidic flow cytometry platform using viscoelastic fluids for polydisperse particle suspensions. / Serhatlioglu, Murat; Jensen, Emil Alstrup; Niora, Maria; Hansen, Anne Todsen; Friberg Nielsen, Christian; Theresia Jansman, Michelle Maria; Hosta-Rigau, Leticia; Dziegiel, Morten Hanefeld; Berg-Sørensen, Kirstine; Hickson, Ian D.; Kristensen, Anders.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 12198, 1219802, 2022.Research output: Contribution to journal › Conference article › Research › peer-review
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TY - GEN
T1 - Development of a fiber-based microfluidic flow cytometry platform using viscoelastic fluids for polydisperse particle suspensions
AU - Serhatlioglu, Murat
AU - Jensen, Emil Alstrup
AU - Niora, Maria
AU - Hansen, Anne Todsen
AU - Friberg Nielsen, Christian
AU - Theresia Jansman, Michelle Maria
AU - Hosta-Rigau, Leticia
AU - Dziegiel, Morten Hanefeld
AU - Berg-Sørensen, Kirstine
AU - Hickson, Ian D.
AU - Kristensen, Anders
N1 - Publisher Copyright: Copyright © 2022 SPIE.
PY - 2022
Y1 - 2022
N2 - Flow cytometry (FC) is a pivotal tool for studying the physical and chemical properties of particles. State-of-the-art FC systems are highly advanced, yet they are expensive, bulky, and require high sample volume, qualified operators, and periodic maintenance. The manipulation of particles suspended in viscoelastic fluids has received increasing attention, especially for miniaturized flow cytometry technologies. This study presents a miniaturized optical capillary FC device using the viscoelastic focusing technique. A straight, one inlet/outlet microcapillary device is precisely aligned to a fiber-coupled laser source and detectors. Forward scattered, side scattered, and fluorescently emitted light signals are collected and analyzed in a real-time environment. The developed platform fits onto an inverted microscope stage enabling real-time microscopy imaging of the particles of interest together with the flow cytometry analysis. We achieved stable viscoelastic focusing and performed FC measurements for rigid polystyrene beads (diameters: 2 - 15 μm), non-spherical human erythrocytes, and canonical shape metaphase human chromosomes. We performed cytometry measurements with a throughput of 100 events/s yielding a coefficient of variation of 2%. This newly developed FC device is a versatile tool and can be operated with any inverted microscope to get the mutual benefits of optical and imaging FC measurements. Furthermore, it is possible to extend these benefits by adding more back-end tools, such as optical trapping and Raman spectroscopy.
AB - Flow cytometry (FC) is a pivotal tool for studying the physical and chemical properties of particles. State-of-the-art FC systems are highly advanced, yet they are expensive, bulky, and require high sample volume, qualified operators, and periodic maintenance. The manipulation of particles suspended in viscoelastic fluids has received increasing attention, especially for miniaturized flow cytometry technologies. This study presents a miniaturized optical capillary FC device using the viscoelastic focusing technique. A straight, one inlet/outlet microcapillary device is precisely aligned to a fiber-coupled laser source and detectors. Forward scattered, side scattered, and fluorescently emitted light signals are collected and analyzed in a real-time environment. The developed platform fits onto an inverted microscope stage enabling real-time microscopy imaging of the particles of interest together with the flow cytometry analysis. We achieved stable viscoelastic focusing and performed FC measurements for rigid polystyrene beads (diameters: 2 - 15 μm), non-spherical human erythrocytes, and canonical shape metaphase human chromosomes. We performed cytometry measurements with a throughput of 100 events/s yielding a coefficient of variation of 2%. This newly developed FC device is a versatile tool and can be operated with any inverted microscope to get the mutual benefits of optical and imaging FC measurements. Furthermore, it is possible to extend these benefits by adding more back-end tools, such as optical trapping and Raman spectroscopy.
KW - blood cells
KW - capillary
KW - chromosomes
KW - flow cytometry
KW - Microfluidics
KW - optofluidics
KW - viscoelastic focusing
U2 - 10.1117/12.2633628
DO - 10.1117/12.2633628
M3 - Conference article
AN - SCOPUS:85140983788
VL - 12198
JO - Progress in Biomedical Optics and Imaging
JF - Progress in Biomedical Optics and Imaging
SN - 1605-7422
M1 - 1219802
T2 - Optical Trapping and Optical Micromanipulation XIX 2022
Y2 - 21 August 2022 through 24 August 2022
ER -
ID: 327140137