Dissolution of the inorganic phase of bone leading to release of calcium regulates osteoclast survival
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Dissolution of the inorganic phase of bone leading to release of calcium regulates osteoclast survival. / Nielsen, Rasmus H; Karsdal, Morten A; Sørensen, Mette G; Dziegiel, Morten Hanefeld; Henriksen, Kim.
In: Biochemical and Biophysical Research Communications, Vol. 360, No. 4, 07.09.2007, p. 834-9.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Dissolution of the inorganic phase of bone leading to release of calcium regulates osteoclast survival
AU - Nielsen, Rasmus H
AU - Karsdal, Morten A
AU - Sørensen, Mette G
AU - Dziegiel, Morten Hanefeld
AU - Henriksen, Kim
PY - 2007/9/7
Y1 - 2007/9/7
N2 - Osteoclasts are the sole cells possessing the ability to resorb calcified bone matrix. This occurs via secretion of hydrochloric acid mediated by the V-ATPase and the chloride channel ClC-7. Loss of acidification leads to osteopetrosis characterized by ablation of bone resorption and increased osteoclast numbers, indicating increased life span of the osteoclasts. To investigate the role of the inorganic phase of bone with respect to osteoclast life span, we used the V-ATPase inhibitor bafilomycin and the calcium uptake antagonist ryanodine on human osteoclasts cultured on calcified and decalcified bone slices. Bafilomycin inhibited bone resorption and increased osteoclast survival on calcified but not decalcified bones. Ryanodine attenuated calcium uptake and thereby augmented osteoclast survival on calcified bones. In summary, we found that acidification leading to calcium release from bone during resorption controls osteoclast survival, potentially explaining the increased numbers of osteoclasts in patients with osteopetrosis.
AB - Osteoclasts are the sole cells possessing the ability to resorb calcified bone matrix. This occurs via secretion of hydrochloric acid mediated by the V-ATPase and the chloride channel ClC-7. Loss of acidification leads to osteopetrosis characterized by ablation of bone resorption and increased osteoclast numbers, indicating increased life span of the osteoclasts. To investigate the role of the inorganic phase of bone with respect to osteoclast life span, we used the V-ATPase inhibitor bafilomycin and the calcium uptake antagonist ryanodine on human osteoclasts cultured on calcified and decalcified bone slices. Bafilomycin inhibited bone resorption and increased osteoclast survival on calcified but not decalcified bones. Ryanodine attenuated calcium uptake and thereby augmented osteoclast survival on calcified bones. In summary, we found that acidification leading to calcium release from bone during resorption controls osteoclast survival, potentially explaining the increased numbers of osteoclasts in patients with osteopetrosis.
KW - Apoptosis
KW - Bone and Bones
KW - Calcium
KW - Cell Survival
KW - Cells, Cultured
KW - Humans
KW - Hydrogen-Ion Concentration
KW - Osteoclasts
U2 - 10.1016/j.bbrc.2007.06.145
DO - 10.1016/j.bbrc.2007.06.145
M3 - Journal article
C2 - 17631274
VL - 360
SP - 834
EP - 839
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -
ID: 47555939