Ficolin-1-PTX3 complex formation promotes clearance of altered self-cells and modulates IL-8 production
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Ficolin-1-PTX3 complex formation promotes clearance of altered self-cells and modulates IL-8 production. / Ma, Ying Jie; Doni, Andrea; Romani, Luigina; Jürgensen, Henrik Jessen; Behrendt, Niels; Mantovani, Alberto; Garred, Peter.
In: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, No. 3, 01.08.2013, p. 1324-33.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Ficolin-1-PTX3 complex formation promotes clearance of altered self-cells and modulates IL-8 production
AU - Ma, Ying Jie
AU - Doni, Andrea
AU - Romani, Luigina
AU - Jürgensen, Henrik Jessen
AU - Behrendt, Niels
AU - Mantovani, Alberto
AU - Garred, Peter
PY - 2013/8/1
Y1 - 2013/8/1
N2 - The long pentraxin 3 (PTX3) has been shown to be important in maintaining internal tissue homeostasis and in protecting against fungal Aspergillus fumigatus infection. However, the molecular mechanisms of how these functions are elicited are poorly delineated. Ficolin-1 is a soluble pattern recognition molecule that interacts with PTX3. We hypothesized that heterocomplexes between ficolin-1 and PTX3 might mediate the signals necessary for sequestration of altered self-cells and A. fumigatus. We were able to show that ficolin-1 interacts with PTX3 via its fibrinogen-like domain. The interaction was affected in a pH- and divalent cation-sensitive manner. The primary binding site for ficolin-1 on PTX3 was located in the N-terminal domain portion of PTX3. Ficolin-1 and PTX3 heterocomplex formation occurred on dying host cells, but not on A. fumigatus. The heterocomplex formation was a prerequisite for enhancement of phagocytosis by human monocyte-derived macrophages and downregulation of IL-8 production during phagocytosis. On A. fumigatus, PTX3 exposed the C-terminal portion of the molecule, probably resulting in steric hindrance of ficolin-1 interaction with PTX3. These results demonstrate that ficolin-1 and PTX3 heterocomplex formation acts as a noninflammatory "find me and eat me" signal to sequester altered-host cells. The fact that the ficolin-1-PTX3 complex formation did not occur on A. fumigatus shows that PTX3 uses different molecular effector mechanisms, depending on which domains it exposes during ligand interaction.
AB - The long pentraxin 3 (PTX3) has been shown to be important in maintaining internal tissue homeostasis and in protecting against fungal Aspergillus fumigatus infection. However, the molecular mechanisms of how these functions are elicited are poorly delineated. Ficolin-1 is a soluble pattern recognition molecule that interacts with PTX3. We hypothesized that heterocomplexes between ficolin-1 and PTX3 might mediate the signals necessary for sequestration of altered self-cells and A. fumigatus. We were able to show that ficolin-1 interacts with PTX3 via its fibrinogen-like domain. The interaction was affected in a pH- and divalent cation-sensitive manner. The primary binding site for ficolin-1 on PTX3 was located in the N-terminal domain portion of PTX3. Ficolin-1 and PTX3 heterocomplex formation occurred on dying host cells, but not on A. fumigatus. The heterocomplex formation was a prerequisite for enhancement of phagocytosis by human monocyte-derived macrophages and downregulation of IL-8 production during phagocytosis. On A. fumigatus, PTX3 exposed the C-terminal portion of the molecule, probably resulting in steric hindrance of ficolin-1 interaction with PTX3. These results demonstrate that ficolin-1 and PTX3 heterocomplex formation acts as a noninflammatory "find me and eat me" signal to sequester altered-host cells. The fact that the ficolin-1-PTX3 complex formation did not occur on A. fumigatus shows that PTX3 uses different molecular effector mechanisms, depending on which domains it exposes during ligand interaction.
KW - Apoptosis
KW - Aspergillosis
KW - Aspergillus fumigatus
KW - Binding Sites
KW - C-Reactive Protein
KW - Cells, Cultured
KW - Humans
KW - Interleukin-8
KW - Lectins
KW - Leukocytes, Mononuclear
KW - Macrophages
KW - Phagocytosis
KW - Protein Binding
KW - Protein Interaction Domains and Motifs
KW - Protein Structure, Tertiary
KW - Serum Amyloid P-Component
KW - Signal Transduction
KW - Surface Plasmon Resonance
U2 - 10.4049/jimmunol.1300382
DO - 10.4049/jimmunol.1300382
M3 - Journal article
C2 - 23817411
VL - 191
SP - 1324
EP - 1333
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 3
ER -
ID: 107122714