Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
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Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo. / Genster, Ninette; Østrup, Olga; Schjalm, Camilla; Eirik Mollnes, Tom; Cowland, Jack B; Garred, Peter.
In: Scientific Reports, Vol. 7, 3852, 2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
AU - Genster, Ninette
AU - Østrup, Olga
AU - Schjalm, Camilla
AU - Eirik Mollnes, Tom
AU - Cowland, Jack B
AU - Garred, Peter
PY - 2017
Y1 - 2017
N2 - Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been suggested. Yet, the contribution of ficolins to inflammatory disease processes remains elusive. To address this, we investigated ficolin deficient mice during a lipopolysaccharide (LPS)-induced model of systemic inflammation. Although murine serum ficolin was shown to bind LPS in vitro, there was no difference between wildtype and ficolin deficient mice in morbidity and mortality by LPS-induced inflammation. Moreover, there was no difference between wildtype and ficolin deficient mice in the inflammatory cytokine profiles after LPS challenge. These findings were substantiated by microarray analysis revealing an unaltered spleen transcriptome profile in ficolin deficient mice compared to wildtype mice. Collectively, results from this study demonstrate that ficolins are not involved in host response to LPS-induced systemic inflammation.
AB - Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been suggested. Yet, the contribution of ficolins to inflammatory disease processes remains elusive. To address this, we investigated ficolin deficient mice during a lipopolysaccharide (LPS)-induced model of systemic inflammation. Although murine serum ficolin was shown to bind LPS in vitro, there was no difference between wildtype and ficolin deficient mice in morbidity and mortality by LPS-induced inflammation. Moreover, there was no difference between wildtype and ficolin deficient mice in the inflammatory cytokine profiles after LPS challenge. These findings were substantiated by microarray analysis revealing an unaltered spleen transcriptome profile in ficolin deficient mice compared to wildtype mice. Collectively, results from this study demonstrate that ficolins are not involved in host response to LPS-induced systemic inflammation.
KW - Journal Article
U2 - 10.1038/s41598-017-04121-w
DO - 10.1038/s41598-017-04121-w
M3 - Journal article
C2 - 28634324
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 3852
ER -
ID: 184836826