Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins
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Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins. / Jürgensen, Henrik J.; Nørregaard, Kirstine S.; Sibree, Megan M.; Santoni‑Rugiu, Eric; Madsen, Daniel H.; Wassilew, Katharina; Krustrup, Dorrit; Garred, Peter; Bugge, Thomas H.; Engelholm, Lars H.; Behrendt, Niels.
In: Journal of Cell Biology, Vol. 218, No. 1, 01.01.2019, p. 333-349.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins
AU - Jürgensen, Henrik J.
AU - Nørregaard, Kirstine S.
AU - Sibree, Megan M.
AU - Santoni‑Rugiu, Eric
AU - Madsen, Daniel H.
AU - Wassilew, Katharina
AU - Krustrup, Dorrit
AU - Garred, Peter
AU - Bugge, Thomas H.
AU - Engelholm, Lars H.
AU - Behrendt, Niels
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Collectins such as mannose-binding lectin (MBL) and surfactant protein D (SP-D) become temporarily deposited in extravascular compartments after tissue injury and perform immune-stimulatory or inflammation-limiting functions. However, their turnover mechanisms, necessary to prevent excessive tissue damage, are virtually unknown. In this study, we show that fibroblasts in injured tissues undertake the clearance of collectins by using the endocytic collagen receptor uPARAP. In cellular assays, several types of collectins were endocytosed in a highly specific uPARAP-dependent process, not shared by the closely related receptor MR/CD206. When introduced into dermis or bleomycin-injured lungs of mice, collectins MBL and SP-D were endocytosed and routed for lysosomal degradation by uPARAP-positive fibroblasts. Fibroblast-specific expression of uPARAP governed endogenous SP-D levels and overall survival after lung injury. In lung tissue from idiopathic pulmonary fibrosis patients, a strong up-regulation of uPARAP was observed in fibroblasts adjacent to regions with SP-D secretion. This study demonstrates a novel immune-regulatory function of fibroblasts and identifies uPARAP as an endocytic receptor in immunity.
AB - Collectins such as mannose-binding lectin (MBL) and surfactant protein D (SP-D) become temporarily deposited in extravascular compartments after tissue injury and perform immune-stimulatory or inflammation-limiting functions. However, their turnover mechanisms, necessary to prevent excessive tissue damage, are virtually unknown. In this study, we show that fibroblasts in injured tissues undertake the clearance of collectins by using the endocytic collagen receptor uPARAP. In cellular assays, several types of collectins were endocytosed in a highly specific uPARAP-dependent process, not shared by the closely related receptor MR/CD206. When introduced into dermis or bleomycin-injured lungs of mice, collectins MBL and SP-D were endocytosed and routed for lysosomal degradation by uPARAP-positive fibroblasts. Fibroblast-specific expression of uPARAP governed endogenous SP-D levels and overall survival after lung injury. In lung tissue from idiopathic pulmonary fibrosis patients, a strong up-regulation of uPARAP was observed in fibroblasts adjacent to regions with SP-D secretion. This study demonstrates a novel immune-regulatory function of fibroblasts and identifies uPARAP as an endocytic receptor in immunity.
U2 - 10.1083/jcb.201802148
DO - 10.1083/jcb.201802148
M3 - Journal article
C2 - 30366943
AN - SCOPUS:85059926686
VL - 218
SP - 333
EP - 349
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 1
ER -
ID: 229443882