In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells

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Standard

In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells. / Nielsen, Leif Kofoed; Norderhaug, Lars; Sandlie, Inger; Dziegiel, Morten Hanefeld.

In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 114, No. 5, 05.2006, p. 345-51.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, LK, Norderhaug, L, Sandlie, I & Dziegiel, MH 2006, 'In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells', APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, vol. 114, no. 5, pp. 345-51. https://doi.org/10.1111/j.1600-0463.2006.apm_381.x

APA

Nielsen, L. K., Norderhaug, L., Sandlie, I., & Dziegiel, M. H. (2006). In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 114(5), 345-51. https://doi.org/10.1111/j.1600-0463.2006.apm_381.x

Vancouver

Nielsen LK, Norderhaug L, Sandlie I, Dziegiel MH. In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2006 May;114(5):345-51. https://doi.org/10.1111/j.1600-0463.2006.apm_381.x

Author

Nielsen, Leif Kofoed ; Norderhaug, Lars ; Sandlie, Inger ; Dziegiel, Morten Hanefeld. / In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells. In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2006 ; Vol. 114, No. 5. pp. 345-51.

Bibtex

@article{495b317284ce4692864ef9f08deab4ae,
title = "In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells",
abstract = "For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti-RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti-RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti-RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti-RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.",
keywords = "Animals, Antibody Specificity, COS Cells, Cercopithecus aethiops, Erythroblastosis, Fetal, Humans, Immunoglobulin G, Luminescent Measurements, Peptides, Plasmids, Protein Engineering, Recombinant Proteins, Respiratory Burst, Rh-Hr Blood-Group System, Rho(D) Immune Globulin, Transfection",
author = "Nielsen, {Leif Kofoed} and Lars Norderhaug and Inger Sandlie and Dziegiel, {Morten Hanefeld}",
year = "2006",
month = may,
doi = "10.1111/j.1600-0463.2006.apm_381.x",
language = "English",
volume = "114",
pages = "345--51",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "5",

}

RIS

TY - JOUR

T1 - In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells

AU - Nielsen, Leif Kofoed

AU - Norderhaug, Lars

AU - Sandlie, Inger

AU - Dziegiel, Morten Hanefeld

PY - 2006/5

Y1 - 2006/5

N2 - For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti-RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti-RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti-RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti-RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.

AB - For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti-RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti-RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti-RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti-RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.

KW - Animals

KW - Antibody Specificity

KW - COS Cells

KW - Cercopithecus aethiops

KW - Erythroblastosis, Fetal

KW - Humans

KW - Immunoglobulin G

KW - Luminescent Measurements

KW - Peptides

KW - Plasmids

KW - Protein Engineering

KW - Recombinant Proteins

KW - Respiratory Burst

KW - Rh-Hr Blood-Group System

KW - Rho(D) Immune Globulin

KW - Transfection

U2 - 10.1111/j.1600-0463.2006.apm_381.x

DO - 10.1111/j.1600-0463.2006.apm_381.x

M3 - Journal article

C2 - 16725010

VL - 114

SP - 345

EP - 351

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 5

ER -

ID: 47556108