Optical and Hydrodynamic Stretching of Single Cells from Blood
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Optical and Hydrodynamic Stretching of Single Cells from Blood. / Thirstrup, Henrik; Rungling, Tony B.; Khalil Al-Hamdani, Mustafa Zyad; Pathanchalinathan, Ragavan; Dziegiel, Morten Hanefeld; Kristensen, Anders; Marie, Rodolphe; Berg-Sørensen, Kirstine.
Optical Trapping Applications, OTA 2017. Vol. Part F64-OTA 2017 The Optical Society (OSA), 2017. OtM4E.1.Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
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TY - GEN
T1 - Optical and Hydrodynamic Stretching of Single Cells from Blood
AU - Thirstrup, Henrik
AU - Rungling, Tony B.
AU - Khalil Al-Hamdani, Mustafa Zyad
AU - Pathanchalinathan, Ragavan
AU - Dziegiel, Morten Hanefeld
AU - Kristensen, Anders
AU - Marie, Rodolphe
AU - Berg-Sørensen, Kirstine
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.
AB - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.
UR - http://www.scopus.com/inward/record.url?scp=85032292055&partnerID=8YFLogxK
U2 - 10.1364/OTA.2017.OtM4E.1
DO - 10.1364/OTA.2017.OtM4E.1
M3 - Article in proceedings
AN - SCOPUS:85032292055
VL - Part F64-OTA 2017
BT - Optical Trapping Applications, OTA 2017
PB - The Optical Society (OSA)
T2 - Optical Trapping Applications, OTA 2017
Y2 - 2 April 2017 through 5 April 2017
ER -
ID: 198566327