TY - JOUR

T1 - Osteoclasts secrete non-bone derived signals that induce bone formation

AU - Karsdal, Morten A

AU - Neutzsky-Wulff, Anita V

AU - Dziegiel, Morten Hanefeld

AU - Christiansen, Claus

AU - Henriksen, Kim

PY - 2008/2/8

Y1 - 2008/2/8

N2 - Bone turnover is a highly regulated process, where bone resorption in the normal healthy individual always is followed by bone formation in a manner referred to as coupling. Patients with osteopetrosis caused by defective acidification of the resorption lacuna have severely decreased resorption, in face of normal or even increased bone formation. This suggests that osteoclasts, not their resorptive activity, are important for sustaining bone formation. To investigate whether osteoclasts mediate control of bone formation by production of bone anabolic signals, we collected conditioned media (CM) from human osteoclasts cultured on either bone or plastic, and tested their effects on bone nodule formation by osteoblasts. Both types of CM were shown to dose-dependently induce bone nodule formation, whereas non-conditioned osteoclast culture medium had no effects. These data show that osteoclasts secrete non-bone derived factors, which induce preosteoblasts to form bone-like nodules, potentially explaining the imbalanced coupling seen in osteopetrotic patients.

AB - Bone turnover is a highly regulated process, where bone resorption in the normal healthy individual always is followed by bone formation in a manner referred to as coupling. Patients with osteopetrosis caused by defective acidification of the resorption lacuna have severely decreased resorption, in face of normal or even increased bone formation. This suggests that osteoclasts, not their resorptive activity, are important for sustaining bone formation. To investigate whether osteoclasts mediate control of bone formation by production of bone anabolic signals, we collected conditioned media (CM) from human osteoclasts cultured on either bone or plastic, and tested their effects on bone nodule formation by osteoblasts. Both types of CM were shown to dose-dependently induce bone nodule formation, whereas non-conditioned osteoclast culture medium had no effects. These data show that osteoclasts secrete non-bone derived factors, which induce preosteoblasts to form bone-like nodules, potentially explaining the imbalanced coupling seen in osteopetrotic patients.

KW - 3T3 Cells

KW - Animals

KW - Cell Communication

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Humans

KW - Mice

KW - Osteoclasts

KW - Osteogenesis

KW - Tissue Engineering

U2 - 10.1016/j.bbrc.2007.11.168

DO - 10.1016/j.bbrc.2007.11.168

M3 - Journal article

C2 - 18068671

VL - 366

SP - 483

EP - 488

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -