Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D. / Dziegiel, Morten Hanefeld; Nielsen, L K; Andersen, P S; Blancher, A; Dickmeiss, E; Engberg, J.

In: Journal of Immunological Methods, Vol. 182, No. 1, 11.05.1995, p. 7-19.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dziegiel, MH, Nielsen, LK, Andersen, PS, Blancher, A, Dickmeiss, E & Engberg, J 1995, 'Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D', Journal of Immunological Methods, vol. 182, no. 1, pp. 7-19.

APA

Dziegiel, M. H., Nielsen, L. K., Andersen, P. S., Blancher, A., Dickmeiss, E., & Engberg, J. (1995). Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D. Journal of Immunological Methods, 182(1), 7-19.

Vancouver

Dziegiel MH, Nielsen LK, Andersen PS, Blancher A, Dickmeiss E, Engberg J. Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D. Journal of Immunological Methods. 1995 May 11;182(1):7-19.

Author

Dziegiel, Morten Hanefeld ; Nielsen, L K ; Andersen, P S ; Blancher, A ; Dickmeiss, E ; Engberg, J. / Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D. In: Journal of Immunological Methods. 1995 ; Vol. 182, No. 1. pp. 7-19.

Bibtex

@article{ab77f46302844d4f9e349ff9e31397be,
title = "Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D",
abstract = "A novel phage display system has been developed for PCR amplification and cloning of the Fab fragments of human immunoglobulin genes. Using this system, we have cloned an antibody from a mouse-human hybridoma cell line directed against the erythrocyte antigen rhesus D. Intact erythrocytes were used for absorption of the Fab phages. Soluble Fab fragments produced from the cloned material showed identical performance to the parental antibody in agglutination assays. Gel filtration confirmed that the Fab fragment consists of a kappa-Fd heterodimer. The successful use of intact cells for selection of specific Fab phages demonstrates that it is possible to by-pass purification of the antigen of interest. Comparison with published germline sequences demonstrated that the immunoglobulin coding regions had the highest homology to the VH 1.9III and V kappa Hum kappa v325 germline genes, respectively.",
keywords = "Amino Acid Sequence, Bacteriophages, Base Sequence, Cloning, Molecular, DNA, Complementary, Genetic Vectors, Humans, Isoantibodies, Molecular Sequence Data, Polymerase Chain Reaction, Recombinant Fusion Proteins, Rh-Hr Blood-Group System",
author = "Dziegiel, {Morten Hanefeld} and Nielsen, {L K} and Andersen, {P S} and A Blancher and E Dickmeiss and J Engberg",
year = "1995",
month = may,
day = "11",
language = "English",
volume = "182",
pages = "7--19",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D

AU - Dziegiel, Morten Hanefeld

AU - Nielsen, L K

AU - Andersen, P S

AU - Blancher, A

AU - Dickmeiss, E

AU - Engberg, J

PY - 1995/5/11

Y1 - 1995/5/11

N2 - A novel phage display system has been developed for PCR amplification and cloning of the Fab fragments of human immunoglobulin genes. Using this system, we have cloned an antibody from a mouse-human hybridoma cell line directed against the erythrocyte antigen rhesus D. Intact erythrocytes were used for absorption of the Fab phages. Soluble Fab fragments produced from the cloned material showed identical performance to the parental antibody in agglutination assays. Gel filtration confirmed that the Fab fragment consists of a kappa-Fd heterodimer. The successful use of intact cells for selection of specific Fab phages demonstrates that it is possible to by-pass purification of the antigen of interest. Comparison with published germline sequences demonstrated that the immunoglobulin coding regions had the highest homology to the VH 1.9III and V kappa Hum kappa v325 germline genes, respectively.

AB - A novel phage display system has been developed for PCR amplification and cloning of the Fab fragments of human immunoglobulin genes. Using this system, we have cloned an antibody from a mouse-human hybridoma cell line directed against the erythrocyte antigen rhesus D. Intact erythrocytes were used for absorption of the Fab phages. Soluble Fab fragments produced from the cloned material showed identical performance to the parental antibody in agglutination assays. Gel filtration confirmed that the Fab fragment consists of a kappa-Fd heterodimer. The successful use of intact cells for selection of specific Fab phages demonstrates that it is possible to by-pass purification of the antigen of interest. Comparison with published germline sequences demonstrated that the immunoglobulin coding regions had the highest homology to the VH 1.9III and V kappa Hum kappa v325 germline genes, respectively.

KW - Amino Acid Sequence

KW - Bacteriophages

KW - Base Sequence

KW - Cloning, Molecular

KW - DNA, Complementary

KW - Genetic Vectors

KW - Humans

KW - Isoantibodies

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

KW - Recombinant Fusion Proteins

KW - Rh-Hr Blood-Group System

M3 - Journal article

C2 - 7769246

VL - 182

SP - 7

EP - 19

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -

ID: 47556849