Phage-display libraries of murine and human antibody Fab fragments

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Phage-display libraries of murine and human antibody Fab fragments. / Engberg, J; Andersen, P S; Nielsen, L K; Dziegiel, Morten Hanefeld; Johansen, L K; Albrechtsen, B.

In: Molecular Biotechnology, Vol. 6, No. 3, 12.1996, p. 287-310.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Engberg, J, Andersen, PS, Nielsen, LK, Dziegiel, MH, Johansen, LK & Albrechtsen, B 1996, 'Phage-display libraries of murine and human antibody Fab fragments', Molecular Biotechnology, vol. 6, no. 3, pp. 287-310. https://doi.org/10.1007/BF02761708

APA

Engberg, J., Andersen, P. S., Nielsen, L. K., Dziegiel, M. H., Johansen, L. K., & Albrechtsen, B. (1996). Phage-display libraries of murine and human antibody Fab fragments. Molecular Biotechnology, 6(3), 287-310. https://doi.org/10.1007/BF02761708

Vancouver

Engberg J, Andersen PS, Nielsen LK, Dziegiel MH, Johansen LK, Albrechtsen B. Phage-display libraries of murine and human antibody Fab fragments. Molecular Biotechnology. 1996 Dec;6(3):287-310. https://doi.org/10.1007/BF02761708

Author

Engberg, J ; Andersen, P S ; Nielsen, L K ; Dziegiel, Morten Hanefeld ; Johansen, L K ; Albrechtsen, B. / Phage-display libraries of murine and human antibody Fab fragments. In: Molecular Biotechnology. 1996 ; Vol. 6, No. 3. pp. 287-310.

Bibtex

@article{8637da6bbe774feabc7fb30fdc9dd4bf,
title = "Phage-display libraries of murine and human antibody Fab fragments",
abstract = "We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.",
keywords = "Animals, Bacteriophages, Gene Library, Humans, Immunoglobulin Fab Fragments, Mice",
author = "J Engberg and Andersen, {P S} and Nielsen, {L K} and Dziegiel, {Morten Hanefeld} and Johansen, {L K} and B Albrechtsen",
year = "1996",
month = dec,
doi = "10.1007/BF02761708",
language = "English",
volume = "6",
pages = "287--310",
journal = "Molecular Biotechnology",
issn = "1073-6085",
publisher = "Humana Press",
number = "3",

}

RIS

TY - JOUR

T1 - Phage-display libraries of murine and human antibody Fab fragments

AU - Engberg, J

AU - Andersen, P S

AU - Nielsen, L K

AU - Dziegiel, Morten Hanefeld

AU - Johansen, L K

AU - Albrechtsen, B

PY - 1996/12

Y1 - 1996/12

N2 - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

AB - We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

KW - Animals

KW - Bacteriophages

KW - Gene Library

KW - Humans

KW - Immunoglobulin Fab Fragments

KW - Mice

U2 - 10.1007/BF02761708

DO - 10.1007/BF02761708

M3 - Journal article

C2 - 9067975

VL - 6

SP - 287

EP - 310

JO - Molecular Biotechnology

JF - Molecular Biotechnology

SN - 1073-6085

IS - 3

ER -

ID: 47556231