Quantitative B-lymphocyte deficiency and increased TCRγδ T-lymphocytes in acute infectious spondylodiscitis
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Quantitative B-lymphocyte deficiency and increased TCRγδ T-lymphocytes in acute infectious spondylodiscitis. / Haugaard, Anna K; Marquart, Hanne V; Kolte, Lilian; Ryder, Lars Peter; Kehrer, Michala; Krogstrup, Maria; Dragsted, Ulrik B; Dahl, Benny; Gjørup, Ida E; Andersen, Åse B; Garred, Peter; Nielsen, Susanne D.
In: Scientific Reports, Vol. 8, 15174, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Quantitative B-lymphocyte deficiency and increased TCRγδ T-lymphocytes in acute infectious spondylodiscitis
AU - Haugaard, Anna K
AU - Marquart, Hanne V
AU - Kolte, Lilian
AU - Ryder, Lars Peter
AU - Kehrer, Michala
AU - Krogstrup, Maria
AU - Dragsted, Ulrik B
AU - Dahl, Benny
AU - Gjørup, Ida E
AU - Andersen, Åse B
AU - Garred, Peter
AU - Nielsen, Susanne D
PY - 2018
Y1 - 2018
N2 - Acute infectious spondylodiscitis (AIS) is a serious infection of the spine with rising incidence and a mortality of 3-6%. The role of the immune system in AIS is largely unknown. We performed extensive B and T-lymphocyte phenotyping in patients with AIS at diagnosis and after treatment cessation. In this prospective multicentre study, flow cytometric analysis of T and B-lymphocyte subsets was performed in 35 patients at diagnosis and 3 months after treatment cessation. We additionally analysed levels of immunoglobulins and IgG subclasses, serum level and genetic variants of mannose-binding lectin, and somatic hypermutation. A total of 22 (61%) patients had B-lymphocytes below reference limit at baseline, persisting in 7 (30%) patients at follow-up. We found a lower proportion of CD19 + CD27 + IgD+ marginal zone B-lymphocytes and a higher proportion of γδ+ T-lymphocyte receptors compared with controls at both time points. Immunoglobulin levels were elevated at baseline compared to follow-up, and not associated with absolute B-lymphocyte count. In conclusion, a large proportion of AIS patients presented with profound B-lymphocyte deficiency, only partly reversible at follow-up. Identification of immune dysfunction related to AIS may allow for future targeted therapeutic interventions to restore host immunity.
AB - Acute infectious spondylodiscitis (AIS) is a serious infection of the spine with rising incidence and a mortality of 3-6%. The role of the immune system in AIS is largely unknown. We performed extensive B and T-lymphocyte phenotyping in patients with AIS at diagnosis and after treatment cessation. In this prospective multicentre study, flow cytometric analysis of T and B-lymphocyte subsets was performed in 35 patients at diagnosis and 3 months after treatment cessation. We additionally analysed levels of immunoglobulins and IgG subclasses, serum level and genetic variants of mannose-binding lectin, and somatic hypermutation. A total of 22 (61%) patients had B-lymphocytes below reference limit at baseline, persisting in 7 (30%) patients at follow-up. We found a lower proportion of CD19 + CD27 + IgD+ marginal zone B-lymphocytes and a higher proportion of γδ+ T-lymphocyte receptors compared with controls at both time points. Immunoglobulin levels were elevated at baseline compared to follow-up, and not associated with absolute B-lymphocyte count. In conclusion, a large proportion of AIS patients presented with profound B-lymphocyte deficiency, only partly reversible at follow-up. Identification of immune dysfunction related to AIS may allow for future targeted therapeutic interventions to restore host immunity.
U2 - 10.1038/s41598-018-33318-w
DO - 10.1038/s41598-018-33318-w
M3 - Journal article
C2 - 30310085
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 15174
ER -
ID: 218085642