Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules

Research output: Contribution to journal › Journal article › Research › peer-review

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Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. / Stravalaci, Matteo; Pagani, Isabel; Paraboschi, Elvezia Maria; Pedotti, Mattia; Doni, Andrea; Scavello, Francesco; Mapelli, Sarah N.; Sironi, Marina; Perucchini, Chiara; Varani, Luca; Matkovic, Milos; Cavalli, Andrea; Cesana, Daniela; Gallina, Pierangela; Pedemonte, Nicoletta; Capurro, Valeria; Clementi, Nicola; Mancini, Nicasio; Invernizzi, Pietro; Bayarri-Olmos, Rafael; Garred, Peter; Rappuoli, Rino; Duga, Stefano; Bottazzi, Barbara; Uguccioni, Mariagrazia; Asselta, Rosanna; Vicenzi, Elisa; Mantovani, Alberto; Garlanda, Cecilia.

In: Nature Immunology, Vol. 23, No. 2, 02.2022, p. 275-286.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Stravalaci, M, Pagani, I, Paraboschi, EM, Pedotti, M, Doni, A, Scavello, F, Mapelli, SN, Sironi, M, Perucchini, C, Varani, L, Matkovic, M, Cavalli, A, Cesana, D, Gallina, P, Pedemonte, N, Capurro, V, Clementi, N, Mancini, N, Invernizzi, P, Bayarri-Olmos, R, Garred, P, Rappuoli, R, Duga, S, Bottazzi, B, Uguccioni, M, Asselta, R, Vicenzi, E, Mantovani, A & Garlanda, C 2022, 'Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules', Nature Immunology, vol. 23, no. 2, pp. 275-286. https://doi.org/10.1038/s41590-021-01114-w

APA

Stravalaci, M., Pagani, I., Paraboschi, E. M., Pedotti, M., Doni, A., Scavello, F., Mapelli, S. N., Sironi, M., Perucchini, C., Varani, L., Matkovic, M., Cavalli, A., Cesana, D., Gallina, P., Pedemonte, N., Capurro, V., Clementi, N., Mancini, N., Invernizzi, P., ... Garlanda, C. (2022). Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. Nature Immunology, 23(2), 275-286. https://doi.org/10.1038/s41590-021-01114-w

Vancouver

Stravalaci M, Pagani I, Paraboschi EM, Pedotti M, Doni A, Scavello F et al. Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. Nature Immunology. 2022 Feb;23(2):275-286. https://doi.org/10.1038/s41590-021-01114-w

Author

Stravalaci, Matteo ; Pagani, Isabel ; Paraboschi, Elvezia Maria ; Pedotti, Mattia ; Doni, Andrea ; Scavello, Francesco ; Mapelli, Sarah N. ; Sironi, Marina ; Perucchini, Chiara ; Varani, Luca ; Matkovic, Milos ; Cavalli, Andrea ; Cesana, Daniela ; Gallina, Pierangela ; Pedemonte, Nicoletta ; Capurro, Valeria ; Clementi, Nicola ; Mancini, Nicasio ; Invernizzi, Pietro ; Bayarri-Olmos, Rafael ; Garred, Peter ; Rappuoli, Rino ; Duga, Stefano ; Bottazzi, Barbara ; Uguccioni, Mariagrazia ; Asselta, Rosanna ; Vicenzi, Elisa ; Mantovani, Alberto ; Garlanda, Cecilia. / Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. In: Nature Immunology. 2022 ; Vol. 23, No. 2. pp. 275-286.

Bibtex

@article{29d871a131e9436a9130281c64543b6e,
title = "Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules",
abstract = "The humoral arm of innate immunity includes diverse molecules with antibody-like functions, some of which serve as disease severity biomarkers in coronavirus disease 2019 (COVID-19). The present study was designed to conduct a systematic investigation of the interaction of human humoral fluid-phase pattern recognition molecules (PRMs) with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Of 12 PRMs tested, the long pentraxin 3 (PTX3) and mannose-binding lectin (MBL) bound the viral nucleocapsid and spike proteins, respectively. MBL bound trimeric spike protein, including that of variants of concern (VoC), in a glycan-dependent manner and inhibited SARS-CoV-2 in three in vitro models. Moreover, after binding to spike protein, MBL activated the lectin pathway of complement activation. Based on retention of glycosylation sites and modeling, MBL was predicted to recognize the Omicron VoC. Genetic polymorphisms at the MBL2 locus were associated with disease severity. These results suggest that selected humoral fluid-phase PRMs can play an important role in resistance to, and pathogenesis of, COVID-19, a finding with translational implications.Stravalaci et al. examined recognition of SARS-CoV-2 by human soluble innate pattern recognition receptor. They report that pentraxin 3 and mannose-binding protein recognize viral nucleoprotein and spike, respectively. Mannose-binding lectin has antiviral activity, and human genetic polymorphisms of MBL2 are associated with more severe COVID-19.",
keywords = "MANNOSE-BINDING LECTIN, LONG PENTRAXIN PTX3, WHOLE-GENOME ASSOCIATION, LENTIVIRAL VECTORS, PROTEIN, POLYMORPHISMS, MBL, AFRICAN, DOMAIN, CELLS",
author = "Matteo Stravalaci and Isabel Pagani and Paraboschi, {Elvezia Maria} and Mattia Pedotti and Andrea Doni and Francesco Scavello and Mapelli, {Sarah N.} and Marina Sironi and Chiara Perucchini and Luca Varani and Milos Matkovic and Andrea Cavalli and Daniela Cesana and Pierangela Gallina and Nicoletta Pedemonte and Valeria Capurro and Nicola Clementi and Nicasio Mancini and Pietro Invernizzi and Rafael Bayarri-Olmos and Peter Garred and Rino Rappuoli and Stefano Duga and Barbara Bottazzi and Mariagrazia Uguccioni and Rosanna Asselta and Elisa Vicenzi and Alberto Mantovani and Cecilia Garlanda",
year = "2022",
month = feb,
doi = "10.1038/s41590-021-01114-w",
language = "English",
volume = "23",
pages = "275--286",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "nature publishing group",
number = "2",

}

RIS

TY - JOUR

T1 - Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules

AU - Stravalaci, Matteo

AU - Pagani, Isabel

AU - Paraboschi, Elvezia Maria

AU - Pedotti, Mattia

AU - Doni, Andrea

AU - Scavello, Francesco

AU - Mapelli, Sarah N.

AU - Sironi, Marina

AU - Perucchini, Chiara

AU - Varani, Luca

AU - Matkovic, Milos

AU - Cavalli, Andrea

AU - Cesana, Daniela

AU - Gallina, Pierangela

AU - Pedemonte, Nicoletta

AU - Capurro, Valeria

AU - Clementi, Nicola

AU - Mancini, Nicasio

AU - Invernizzi, Pietro

AU - Bayarri-Olmos, Rafael

AU - Garred, Peter

AU - Rappuoli, Rino

AU - Duga, Stefano

AU - Bottazzi, Barbara

AU - Uguccioni, Mariagrazia

AU - Asselta, Rosanna

AU - Vicenzi, Elisa

AU - Mantovani, Alberto

AU - Garlanda, Cecilia

PY - 2022/2

Y1 - 2022/2

N2 - The humoral arm of innate immunity includes diverse molecules with antibody-like functions, some of which serve as disease severity biomarkers in coronavirus disease 2019 (COVID-19). The present study was designed to conduct a systematic investigation of the interaction of human humoral fluid-phase pattern recognition molecules (PRMs) with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Of 12 PRMs tested, the long pentraxin 3 (PTX3) and mannose-binding lectin (MBL) bound the viral nucleocapsid and spike proteins, respectively. MBL bound trimeric spike protein, including that of variants of concern (VoC), in a glycan-dependent manner and inhibited SARS-CoV-2 in three in vitro models. Moreover, after binding to spike protein, MBL activated the lectin pathway of complement activation. Based on retention of glycosylation sites and modeling, MBL was predicted to recognize the Omicron VoC. Genetic polymorphisms at the MBL2 locus were associated with disease severity. These results suggest that selected humoral fluid-phase PRMs can play an important role in resistance to, and pathogenesis of, COVID-19, a finding with translational implications.Stravalaci et al. examined recognition of SARS-CoV-2 by human soluble innate pattern recognition receptor. They report that pentraxin 3 and mannose-binding protein recognize viral nucleoprotein and spike, respectively. Mannose-binding lectin has antiviral activity, and human genetic polymorphisms of MBL2 are associated with more severe COVID-19.

AB - The humoral arm of innate immunity includes diverse molecules with antibody-like functions, some of which serve as disease severity biomarkers in coronavirus disease 2019 (COVID-19). The present study was designed to conduct a systematic investigation of the interaction of human humoral fluid-phase pattern recognition molecules (PRMs) with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Of 12 PRMs tested, the long pentraxin 3 (PTX3) and mannose-binding lectin (MBL) bound the viral nucleocapsid and spike proteins, respectively. MBL bound trimeric spike protein, including that of variants of concern (VoC), in a glycan-dependent manner and inhibited SARS-CoV-2 in three in vitro models. Moreover, after binding to spike protein, MBL activated the lectin pathway of complement activation. Based on retention of glycosylation sites and modeling, MBL was predicted to recognize the Omicron VoC. Genetic polymorphisms at the MBL2 locus were associated with disease severity. These results suggest that selected humoral fluid-phase PRMs can play an important role in resistance to, and pathogenesis of, COVID-19, a finding with translational implications.Stravalaci et al. examined recognition of SARS-CoV-2 by human soluble innate pattern recognition receptor. They report that pentraxin 3 and mannose-binding protein recognize viral nucleoprotein and spike, respectively. Mannose-binding lectin has antiviral activity, and human genetic polymorphisms of MBL2 are associated with more severe COVID-19.

KW - MANNOSE-BINDING LECTIN

KW - LONG PENTRAXIN PTX3

KW - WHOLE-GENOME ASSOCIATION

KW - LENTIVIRAL VECTORS

KW - PROTEIN

KW - POLYMORPHISMS

KW - MBL

KW - AFRICAN

KW - DOMAIN

KW - CELLS

U2 - 10.1038/s41590-021-01114-w

DO - 10.1038/s41590-021-01114-w

M3 - Journal article

C2 - 35102342

VL - 23

SP - 275

EP - 286

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 2

ER -

ID: 314957766