Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2years of screening experience from Denmark
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Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2years of screening experience from Denmark. / Clausen, F. Banch; Steffensen, R.; Christiansen, M.; Rudby, M.; Jakobsen, M. A.; Jakobsen, T. R.; Krog, G. R.; Madsen, R. D.; Nielsen, K. R.; Rieneck, K.; Sprogøe, U.; Homburg, K. M.; Baech, J.; Dziegiel, M. H.; Grunnet, N.
In: Prenatal Diagnosis, Vol. 34, No. 10, 2014, p. 1000-1005.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Routine noninvasive prenatal screening for fetal RHD in plasma of RhD-negative pregnant women-2years of screening experience from Denmark
AU - Clausen, F. Banch
AU - Steffensen, R.
AU - Christiansen, M.
AU - Rudby, M.
AU - Jakobsen, M. A.
AU - Jakobsen, T. R.
AU - Krog, G. R.
AU - Madsen, R. D.
AU - Nielsen, K. R.
AU - Rieneck, K.
AU - Sprogøe, U.
AU - Homburg, K. M.
AU - Baech, J.
AU - Dziegiel, M. H.
AU - Grunnet, N.
PY - 2014
Y1 - 2014
N2 - Objective: Prenatal and postnatal RhD prophylaxis reduces the risk of RhD immunization in pregnancies of RhD-negative women. Based on the result from prenatal screening for the fetal RHD gene, prenatal RhD prophylaxis in Denmark is targeted to RhD-negative women who carry an RhD-positive fetus. Here, we present a 2-year evaluation of a nationwide prenatal RHD screening. Methods: Blood samples were drawn from RhD-negative women in gestational week 25. DNA was extracted from maternal plasma and analyzed for the RHD gene. The prenatal RHD results were compared with the serological typing of newborns in 12,668 pregnancies. Early compliance was assessed for 690 pregnancies. Results: The sensitivity for the detection of fetal RHD was 99.9% (95% CI: 99.7-99.9%). Unnecessary recommendation of prenatal RhD prophylaxis was avoided in 97.3% of the women carrying an RhD-negative fetus. Fetuses that were seropositive for RhD were not detected in 11 pregnancies (0.087%). The sample uptake percentage was 84.2%, and the compliance for prenatal anti-D administration was 93.2%. Conclusion: The high sensitivity, maintained over 2years, underlines the reliability of routine prenatal fetal RHD screening in RhD-negative pregnant women, specifically at 25weeks of gestation. The remaining challenges are logistical and are related to program compliance.
AB - Objective: Prenatal and postnatal RhD prophylaxis reduces the risk of RhD immunization in pregnancies of RhD-negative women. Based on the result from prenatal screening for the fetal RHD gene, prenatal RhD prophylaxis in Denmark is targeted to RhD-negative women who carry an RhD-positive fetus. Here, we present a 2-year evaluation of a nationwide prenatal RHD screening. Methods: Blood samples were drawn from RhD-negative women in gestational week 25. DNA was extracted from maternal plasma and analyzed for the RHD gene. The prenatal RHD results were compared with the serological typing of newborns in 12,668 pregnancies. Early compliance was assessed for 690 pregnancies. Results: The sensitivity for the detection of fetal RHD was 99.9% (95% CI: 99.7-99.9%). Unnecessary recommendation of prenatal RhD prophylaxis was avoided in 97.3% of the women carrying an RhD-negative fetus. Fetuses that were seropositive for RhD were not detected in 11 pregnancies (0.087%). The sample uptake percentage was 84.2%, and the compliance for prenatal anti-D administration was 93.2%. Conclusion: The high sensitivity, maintained over 2years, underlines the reliability of routine prenatal fetal RHD screening in RhD-negative pregnant women, specifically at 25weeks of gestation. The remaining challenges are logistical and are related to program compliance.
U2 - 10.1002/pd.4419
DO - 10.1002/pd.4419
M3 - Journal article
C2 - 24860987
AN - SCOPUS:84908232413
VL - 34
SP - 1000
EP - 1005
JO - Prenatal Diagnosis
JF - Prenatal Diagnosis
SN - 0197-3851
IS - 10
ER -
ID: 186449955