SARS-CoV-2 antibody responses are correlated to disease severity in COVID-19 convalescent individuals
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SARS-CoV-2 antibody responses are correlated to disease severity in COVID-19 convalescent individuals. / Hansen, Cecilie Bo; Jarlhelt, Ida; Pérez-Alós, Laura; Landsy, Lone Hummelshøj; Loftager, Mette; Rosbjerg, Anne; Helgstrand, Charlotte; Bjelke, Jais Rose; Egebjerg, Thomas; Jardine, Joseph G.; Jørgensen, Charlotte Sværke; Iversen, Kasper; Bayarri-Olmos, Rafael; Garred, Peter; Skjoedt, Mikkel Ole.
In: Journal of Immunology, Vol. 206, No. 1, 2021, p. 109-117.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - SARS-CoV-2 antibody responses are correlated to disease severity in COVID-19 convalescent individuals
AU - Hansen, Cecilie Bo
AU - Jarlhelt, Ida
AU - Pérez-Alós, Laura
AU - Landsy, Lone Hummelshøj
AU - Loftager, Mette
AU - Rosbjerg, Anne
AU - Helgstrand, Charlotte
AU - Bjelke, Jais Rose
AU - Egebjerg, Thomas
AU - Jardine, Joseph G.
AU - Jørgensen, Charlotte Sværke
AU - Iversen, Kasper
AU - Bayarri-Olmos, Rafael
AU - Garred, Peter
AU - Skjoedt, Mikkel Ole
PY - 2021
Y1 - 2021
N2 - Globally, the COVID-19 pandemic has had extreme consequences for the healthcare system and has led to calls for diagnostic tools to monitor and understand the transmission, pathogenesis, and epidemiology, as well as to evaluate future vaccination strategies. In this study, we have developed novel, to our knowledge, flexible ELISA-based assays for specific detection of human SARS-CoV-2 Abs against the receptor-binding domain, including an Ag sandwich ELISA relevant for large population screening and three isotype-specific assays for in-depth diagnostics. Their performance was evaluated in a cohort of 350 convalescent participants with previous COVID-19 infection, ranging from asymptomatic to critical cases. We mapped the Ab responses to different areas on protein N and S and showed that the IgM, A, and G Ab responses against receptor-binding domain are significantly correlated to the disease severity. These assays and the data generated from them are highly relevant for diagnostics and prognostics and contribute to the understanding of long-term COVID-19 immunity.
AB - Globally, the COVID-19 pandemic has had extreme consequences for the healthcare system and has led to calls for diagnostic tools to monitor and understand the transmission, pathogenesis, and epidemiology, as well as to evaluate future vaccination strategies. In this study, we have developed novel, to our knowledge, flexible ELISA-based assays for specific detection of human SARS-CoV-2 Abs against the receptor-binding domain, including an Ag sandwich ELISA relevant for large population screening and three isotype-specific assays for in-depth diagnostics. Their performance was evaluated in a cohort of 350 convalescent participants with previous COVID-19 infection, ranging from asymptomatic to critical cases. We mapped the Ab responses to different areas on protein N and S and showed that the IgM, A, and G Ab responses against receptor-binding domain are significantly correlated to the disease severity. These assays and the data generated from them are highly relevant for diagnostics and prognostics and contribute to the understanding of long-term COVID-19 immunity.
U2 - 10.4049/jimmunol.2000898
DO - 10.4049/jimmunol.2000898
M3 - Journal article
C2 - 33208457
AN - SCOPUS:85098690997
VL - 206
SP - 109
EP - 117
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 1
ER -
ID: 254998934