The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells
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The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells. / Odum, Niels; Bregenholt, S; Eriksen, K W; Skov, S; Ryder, Lars P.; Bendtzen, Klaus; Van Neerven, R J; Svejgaard, A; Garred, P.
In: HLA, Vol. 54, No. 6, 1999, p. 572-7.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells
AU - Odum, Niels
AU - Bregenholt, S
AU - Eriksen, K W
AU - Skov, S
AU - Ryder, Lars P.
AU - Bendtzen, Klaus
AU - Van Neerven, R J
AU - Svejgaard, A
AU - Garred, P
N1 - Keywords: Biological Markers; Cell Differentiation; Cell Lineage; Cells, Cultured; Flow Cytometry; Humans; Receptors, CCR5; Th1 Cells
PY - 1999
Y1 - 1999
N2 - The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.
AB - The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.
U2 - 10.1034/j.1399-0039.1999.540606.x
DO - 10.1034/j.1399-0039.1999.540606.x
M3 - Journal article
C2 - 10674971
VL - 54
SP - 572
EP - 577
JO - HLA
JF - HLA
SN - 2059-2302
IS - 6
ER -
ID: 10617426