The development and characterization of an ELISA specifically detecting the active form of cathepsin K

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The development and characterization of an ELISA specifically detecting the active form of cathepsin K. / Sun, S; Karsdal, M A; Bay-Jensen, A C; Sørensen, M G; Zheng, Q; Dziegiel, Morten Hanefeld; Maksymowych, W P; Henriksen, K.

In: Clinical Biochemistry, 24.04.2013.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sun, S, Karsdal, MA, Bay-Jensen, AC, Sørensen, MG, Zheng, Q, Dziegiel, MH, Maksymowych, WP & Henriksen, K 2013, 'The development and characterization of an ELISA specifically detecting the active form of cathepsin K', Clinical Biochemistry. https://doi.org/10.1016/j.clinbiochem.2013.04.012

APA

Sun, S., Karsdal, M. A., Bay-Jensen, A. C., Sørensen, M. G., Zheng, Q., Dziegiel, M. H., Maksymowych, W. P., & Henriksen, K. (2013). The development and characterization of an ELISA specifically detecting the active form of cathepsin K. Clinical Biochemistry. https://doi.org/10.1016/j.clinbiochem.2013.04.012

Vancouver

Sun S, Karsdal MA, Bay-Jensen AC, Sørensen MG, Zheng Q, Dziegiel MH et al. The development and characterization of an ELISA specifically detecting the active form of cathepsin K. Clinical Biochemistry. 2013 Apr 24. https://doi.org/10.1016/j.clinbiochem.2013.04.012

Author

Sun, S ; Karsdal, M A ; Bay-Jensen, A C ; Sørensen, M G ; Zheng, Q ; Dziegiel, Morten Hanefeld ; Maksymowych, W P ; Henriksen, K. / The development and characterization of an ELISA specifically detecting the active form of cathepsin K. In: Clinical Biochemistry. 2013.

Bibtex

@article{913970d381bd423ea356193cbe2dafeb,

title = "The development and characterization of an ELISA specifically detecting the active form of cathepsin K",

abstract = "OBJECTIVE: Cathepsin K plays essential roles in bone resorption and is intensely investigated as a therapeutic target for the treatment of osteoporosis. Hence an assessment of the active form of cathepsin K may provide important biological information in metabolic bone diseases, such as osteoporosis or ankylosing spondylitis. METHODS: Presently there are no robust assays for the assessment of active cathepsin K in serum, and therefore an ELISA specifically detecting the N-terminal of the active form of cathepsin K was developed. RESULTS: The assay was technically robust, with a lowest limit of detection (LOD) of 0.085ng/mL. The average intra- and inter-assay CV% were 6.60% and 8.56% respectively. The dilution recovery and spike recovery tests in human serum were within 100±20% within the range of the assay. A comparison of latent and active cathepsin K confirmed specificity towards the active form. Quantification of the levels of active cathepsin K in supernatants of purified human osteoclasts compared to corresponding macrophages showed a 30-fold induction (p",

author = "S Sun and Karsdal, {M A} and Bay-Jensen, {A C} and S{\o}rensen, {M G} and Q Zheng and Dziegiel, {Morten Hanefeld} and Maksymowych, {W P} and K Henriksen",

year = "2013",

month = apr,

day = "24",

doi = "10.1016/j.clinbiochem.2013.04.012",

language = "English",

journal = "Clinical Biochemistry",

issn = "0009-9120",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - The development and characterization of an ELISA specifically detecting the active form of cathepsin K

AU - Sun, S

AU - Karsdal, M A

AU - Bay-Jensen, A C

AU - Sørensen, M G

AU - Zheng, Q

AU - Dziegiel, Morten Hanefeld

AU - Maksymowych, W P

AU - Henriksen, K

PY - 2013/4/24

Y1 - 2013/4/24

N2 - OBJECTIVE: Cathepsin K plays essential roles in bone resorption and is intensely investigated as a therapeutic target for the treatment of osteoporosis. Hence an assessment of the active form of cathepsin K may provide important biological information in metabolic bone diseases, such as osteoporosis or ankylosing spondylitis. METHODS: Presently there are no robust assays for the assessment of active cathepsin K in serum, and therefore an ELISA specifically detecting the N-terminal of the active form of cathepsin K was developed. RESULTS: The assay was technically robust, with a lowest limit of detection (LOD) of 0.085ng/mL. The average intra- and inter-assay CV% were 6.60% and 8.56% respectively. The dilution recovery and spike recovery tests in human serum were within 100±20% within the range of the assay. A comparison of latent and active cathepsin K confirmed specificity towards the active form. Quantification of the levels of active cathepsin K in supernatants of purified human osteoclasts compared to corresponding macrophages showed a 30-fold induction (p

AB - OBJECTIVE: Cathepsin K plays essential roles in bone resorption and is intensely investigated as a therapeutic target for the treatment of osteoporosis. Hence an assessment of the active form of cathepsin K may provide important biological information in metabolic bone diseases, such as osteoporosis or ankylosing spondylitis. METHODS: Presently there are no robust assays for the assessment of active cathepsin K in serum, and therefore an ELISA specifically detecting the N-terminal of the active form of cathepsin K was developed. RESULTS: The assay was technically robust, with a lowest limit of detection (LOD) of 0.085ng/mL. The average intra- and inter-assay CV% were 6.60% and 8.56% respectively. The dilution recovery and spike recovery tests in human serum were within 100±20% within the range of the assay. A comparison of latent and active cathepsin K confirmed specificity towards the active form. Quantification of the levels of active cathepsin K in supernatants of purified human osteoclasts compared to corresponding macrophages showed a 30-fold induction (p

U2 - 10.1016/j.clinbiochem.2013.04.012

DO - 10.1016/j.clinbiochem.2013.04.012

M3 - Journal article

C2 - 23623829

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

ER -

ID: 47554597

Department of Clinical Medicine