Urea and water are transported through different pathways in the red blood cell membrane
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Urea and water are transported through different pathways in the red blood cell membrane. / Brahm, Jesper; Dziegiel, Morten Hanefeld; Leifelt, Jonas.
In: The Journal of general physiology, Vol. 155, No. 8, e202213322, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Urea and water are transported through different pathways in the red blood cell membrane
AU - Brahm, Jesper
AU - Dziegiel, Morten Hanefeld
AU - Leifelt, Jonas
N1 - Publisher Copyright: © 2023 Brahm et al.
PY - 2023
Y1 - 2023
N2 - Several studies of the urea transporter UT-B expressed in Xenopus oocytes and in genetically modified red blood cells (RBC) have concluded that UT-B also transports water. In the present study, we use unmodified RBC to test that conclusion. We find that the permeability of urea, Pu (cm/s), has a 10-fold donor variation, while the diffusional water permeability, Pd (cm/s), remains unchanged. Additionally, we observe that phloretin inhibits Pu but not Pd, and that the time course of maximum p-chloromercuribenzosulfonate inhibition of Pu and Pd differs-Pu inhibition takes <2 min, whereas Pd inhibition requires ≥1 h of incubation. The findings in the present study are in line with a previous comparative study using unmodified RBC from four animals and a solvent drag study using human RBC, and they lead us to reject the conclusion that the UT-B transporter represents a common pathway for both solutes.
AB - Several studies of the urea transporter UT-B expressed in Xenopus oocytes and in genetically modified red blood cells (RBC) have concluded that UT-B also transports water. In the present study, we use unmodified RBC to test that conclusion. We find that the permeability of urea, Pu (cm/s), has a 10-fold donor variation, while the diffusional water permeability, Pd (cm/s), remains unchanged. Additionally, we observe that phloretin inhibits Pu but not Pd, and that the time course of maximum p-chloromercuribenzosulfonate inhibition of Pu and Pd differs-Pu inhibition takes <2 min, whereas Pd inhibition requires ≥1 h of incubation. The findings in the present study are in line with a previous comparative study using unmodified RBC from four animals and a solvent drag study using human RBC, and they lead us to reject the conclusion that the UT-B transporter represents a common pathway for both solutes.
U2 - 10.1085/jgp.202213322
DO - 10.1085/jgp.202213322
M3 - Journal article
C2 - 37389569
AN - SCOPUS:85164234071
VL - 155
JO - Journal of General Physiology
JF - Journal of General Physiology
SN - 0022-1295
IS - 8
M1 - e202213322
ER -
ID: 362144500