Characterisation of Carbapenemase-producing Acinetobacter baumannii Isolates from Danish Patients 2014–2021: Detection of a New International Clone – IC11
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Characterisation of Carbapenemase-producing Acinetobacter baumannii Isolates from Danish Patients 2014–2021 : Detection of a New International Clone – IC11. / Hansen, Frank; Porsbo, Lone Jannok; Frandsen, Tove Havnhøj; Kaygisiz, Ayşe Nur Sarı; Roer, Louise; Henius, Anna E.; Holzknecht, Barbara Juliane; Søes, Lillian; Schønning, Kristian; Røder, Bent L.; Justesen, Ulrik S.; Østergaard, Claus; Dzajic, Esad; Wang, Mikala; Ank, Nina; Higgins, Paul G.; Hasman, Henrik; Hammerum, Anette M.
In: International Journal of Antimicrobial Agents, Vol. 62, No. 2, 106866, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Characterisation of Carbapenemase-producing Acinetobacter baumannii Isolates from Danish Patients 2014–2021
T2 - Detection of a New International Clone – IC11
AU - Hansen, Frank
AU - Porsbo, Lone Jannok
AU - Frandsen, Tove Havnhøj
AU - Kaygisiz, Ayşe Nur Sarı
AU - Roer, Louise
AU - Henius, Anna E.
AU - Holzknecht, Barbara Juliane
AU - Søes, Lillian
AU - Schønning, Kristian
AU - Røder, Bent L.
AU - Justesen, Ulrik S.
AU - Østergaard, Claus
AU - Dzajic, Esad
AU - Wang, Mikala
AU - Ank, Nina
AU - Higgins, Paul G.
AU - Hasman, Henrik
AU - Hammerum, Anette M.
N1 - Publisher Copyright: © 2023 The Author(s)
PY - 2023
Y1 - 2023
N2 - Objectives: This study aimed to characterise carbapenemase-producing Acinetobacter baumannii (A. baumannii) isolates from Danish patients using whole genome sequencing (WGS). It also compared typing and epidemiological data for further investigation of the spread and origin of the carbapenemase-producing A. baumannii isolates. Methods: From 1 January 2014 to 30 September 2021, 141 carbapenemase-producing A. baumannii isolates, received at the national reference laboratory at Statens Serum Institut, were investigated using WGS. Multilocus sequence typing (MLST) and cgMLST data, obtained by SeqSphere+ software, were linked to data related to source of isolation, patient age and sex, hospital admission and travel history. Results: Most of the carbapenemase-producing A. baumannii isolates were from males (n = 100, 71%). Most patients (n = 88, 63%) had travelled outside Scandinavia before admission to a Danish hospital. The most prevalent carbapenemase gene was blaOXA-23 (n = 124). Isolates belonging to the dominating international clone IC2 accounted for 78% of all isolates. A new international ST164/OXA-91 clone, proposed to be named IC11, was recognised and described. The cgMLST analysis revealed 17 clusters, reflecting both sporadic travel to similar geographical areas and confirmed outbreaks in Danish hospitals. Conclusions: The occurrence of carbapenemase-producing A. baumannii in Denmark was still low; however, isolates belonging to major international clones with a high potential to spread within hospitals, mainly IC2, dominated. OXA-23 was by far the most prevalent carbapenemase detected. Sporadic and travel-related introductions to Danish hospitals, also intra-hospital transmission, could be confirmed, emphasising the need for continuing vigilance.
AB - Objectives: This study aimed to characterise carbapenemase-producing Acinetobacter baumannii (A. baumannii) isolates from Danish patients using whole genome sequencing (WGS). It also compared typing and epidemiological data for further investigation of the spread and origin of the carbapenemase-producing A. baumannii isolates. Methods: From 1 January 2014 to 30 September 2021, 141 carbapenemase-producing A. baumannii isolates, received at the national reference laboratory at Statens Serum Institut, were investigated using WGS. Multilocus sequence typing (MLST) and cgMLST data, obtained by SeqSphere+ software, were linked to data related to source of isolation, patient age and sex, hospital admission and travel history. Results: Most of the carbapenemase-producing A. baumannii isolates were from males (n = 100, 71%). Most patients (n = 88, 63%) had travelled outside Scandinavia before admission to a Danish hospital. The most prevalent carbapenemase gene was blaOXA-23 (n = 124). Isolates belonging to the dominating international clone IC2 accounted for 78% of all isolates. A new international ST164/OXA-91 clone, proposed to be named IC11, was recognised and described. The cgMLST analysis revealed 17 clusters, reflecting both sporadic travel to similar geographical areas and confirmed outbreaks in Danish hospitals. Conclusions: The occurrence of carbapenemase-producing A. baumannii in Denmark was still low; however, isolates belonging to major international clones with a high potential to spread within hospitals, mainly IC2, dominated. OXA-23 was by far the most prevalent carbapenemase detected. Sporadic and travel-related introductions to Danish hospitals, also intra-hospital transmission, could be confirmed, emphasising the need for continuing vigilance.
KW - cgMLST
KW - IC2
KW - MLST
KW - OXA-23
KW - WGS
UR - http://www.scopus.com/inward/record.url?scp=85161657986&partnerID=8YFLogxK
U2 - 10.1016/j.ijantimicag.2023.106866
DO - 10.1016/j.ijantimicag.2023.106866
M3 - Journal article
C2 - 37244424
AN - SCOPUS:85161657986
VL - 62
JO - International Journal of Antimicrobial Agents
JF - International Journal of Antimicrobial Agents
SN - 0924-8579
IS - 2
M1 - 106866
ER -
ID: 367701551