The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity

Research output: Contribution to journalJournal articleResearchpeer-review

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The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity. / Fonnes, Siv; Holzknecht, Barbara Juliane; Gasbjerg, Lærke Smidt; Weisser, Johan Juhl; Hallberg, Hanne Wiese; Arpi, Magnus; Rosenberg, Jacob.

In: Drug Research, Vol. 68, No. 6, 2018, p. 349-354.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Fonnes, S, Holzknecht, BJ, Gasbjerg, LS, Weisser, JJ, Hallberg, HW, Arpi, M & Rosenberg, J 2018, 'The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity', Drug Research, vol. 68, no. 6, pp. 349-354. https://doi.org/10.1055/s-0043-123933

APA

Fonnes, S., Holzknecht, B. J., Gasbjerg, L. S., Weisser, J. J., Hallberg, H. W., Arpi, M., & Rosenberg, J. (2018). The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity. Drug Research, 68(6), 349-354. https://doi.org/10.1055/s-0043-123933

Vancouver

Fonnes S, Holzknecht BJ, Gasbjerg LS, Weisser JJ, Hallberg HW, Arpi M et al. The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity. Drug Research. 2018;68(6):349-354. https://doi.org/10.1055/s-0043-123933

Author

Fonnes, Siv ; Holzknecht, Barbara Juliane ; Gasbjerg, Lærke Smidt ; Weisser, Johan Juhl ; Hallberg, Hanne Wiese ; Arpi, Magnus ; Rosenberg, Jacob. / The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity. In: Drug Research. 2018 ; Vol. 68, No. 6. pp. 349-354.

Bibtex

@article{7698a305f48b45178dad6a95f2535f40,
title = "The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity",
abstract = "BACKGROUND: Treatment of secondary peritonitis includes surgery and antimicrobial agents. Antimicrobial agents are often administered intravenously, however, the alternative route intraperitoneal administration could be considered. Investigations must be conducted prior to clinical application. Therefore, we aimed to investigate the combination of fosfomycin, metronidazole, and recombinant human granulocyte-macrophage colony-stimulating factor with regard to its chemical properties and the solution's stability. In addition, the antibacterial effect of the mixed drug solution was compared with the effect of the individual antibacterial agents.METHODS: The drugs were mixed to an aqueous solution. Basic chemical investigations of pH, precipitation, and calculated osmolarity of the drug combination were conducted. Fosfomycin and metronidazole's chemical stability was investigated using High Pressure Liquid Chromatography-Mass Spectrometry. Microbiological investigations using the agar cup method were carried out to measure the antibacterial effect of fosfomycin and metronidazole.RESULTS: The aqueous solution of the combination of the three drugs had a pH of 7.46-7.62, which was stable during 24 h, was without precipitation, and had a calculated osmolarity of 293 mOsm/l. High Pressure Liquid Chromatography-Mass Spectrometry found stable concentrations of fosfomycin and metronidazole both alone and in combination during 24 h. The antibacterial effect of the drug combination solution was similar to the antibacterial effects of fosfomycin and metronidazole alone.CONCLUSION: The drug combination had neutral and stable pH, was iso-osmotic, and had stable concentrations during 24 h of storage. The antibacterial effect of fosfomycin and metronidazole were not altered when the drugs were mixed.",
keywords = "Anti-Bacterial Agents/pharmacology, Drug Combinations, Drug Stability, Fosfomycin/pharmacology, Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology, Humans, Metronidazole/pharmacology, Microbial Sensitivity Tests, Recombinant Proteins/pharmacology, chemistry, inflammation, Antibacterial drugs",
author = "Siv Fonnes and Holzknecht, {Barbara Juliane} and Gasbjerg, {L{\ae}rke Smidt} and Weisser, {Johan Juhl} and Hallberg, {Hanne Wiese} and Magnus Arpi and Jacob Rosenberg",
note = "{\textcopyright} Georg Thieme Verlag KG Stuttgart · New York.",
year = "2018",
doi = "10.1055/s-0043-123933",
language = "English",
volume = "68",
pages = "349--354",
journal = "Drug Research",
issn = "2194-9379",
publisher = "GeorgThieme Verlag",
number = "6",

}

RIS

TY - JOUR

T1 - The Combination of Fosfomycin, Metronidazole, and Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is Stable in vitro and Has Maintained Antibacterial Activity

AU - Fonnes, Siv

AU - Holzknecht, Barbara Juliane

AU - Gasbjerg, Lærke Smidt

AU - Weisser, Johan Juhl

AU - Hallberg, Hanne Wiese

AU - Arpi, Magnus

AU - Rosenberg, Jacob

N1 - © Georg Thieme Verlag KG Stuttgart · New York.

PY - 2018

Y1 - 2018

N2 - BACKGROUND: Treatment of secondary peritonitis includes surgery and antimicrobial agents. Antimicrobial agents are often administered intravenously, however, the alternative route intraperitoneal administration could be considered. Investigations must be conducted prior to clinical application. Therefore, we aimed to investigate the combination of fosfomycin, metronidazole, and recombinant human granulocyte-macrophage colony-stimulating factor with regard to its chemical properties and the solution's stability. In addition, the antibacterial effect of the mixed drug solution was compared with the effect of the individual antibacterial agents.METHODS: The drugs were mixed to an aqueous solution. Basic chemical investigations of pH, precipitation, and calculated osmolarity of the drug combination were conducted. Fosfomycin and metronidazole's chemical stability was investigated using High Pressure Liquid Chromatography-Mass Spectrometry. Microbiological investigations using the agar cup method were carried out to measure the antibacterial effect of fosfomycin and metronidazole.RESULTS: The aqueous solution of the combination of the three drugs had a pH of 7.46-7.62, which was stable during 24 h, was without precipitation, and had a calculated osmolarity of 293 mOsm/l. High Pressure Liquid Chromatography-Mass Spectrometry found stable concentrations of fosfomycin and metronidazole both alone and in combination during 24 h. The antibacterial effect of the drug combination solution was similar to the antibacterial effects of fosfomycin and metronidazole alone.CONCLUSION: The drug combination had neutral and stable pH, was iso-osmotic, and had stable concentrations during 24 h of storage. The antibacterial effect of fosfomycin and metronidazole were not altered when the drugs were mixed.

AB - BACKGROUND: Treatment of secondary peritonitis includes surgery and antimicrobial agents. Antimicrobial agents are often administered intravenously, however, the alternative route intraperitoneal administration could be considered. Investigations must be conducted prior to clinical application. Therefore, we aimed to investigate the combination of fosfomycin, metronidazole, and recombinant human granulocyte-macrophage colony-stimulating factor with regard to its chemical properties and the solution's stability. In addition, the antibacterial effect of the mixed drug solution was compared with the effect of the individual antibacterial agents.METHODS: The drugs were mixed to an aqueous solution. Basic chemical investigations of pH, precipitation, and calculated osmolarity of the drug combination were conducted. Fosfomycin and metronidazole's chemical stability was investigated using High Pressure Liquid Chromatography-Mass Spectrometry. Microbiological investigations using the agar cup method were carried out to measure the antibacterial effect of fosfomycin and metronidazole.RESULTS: The aqueous solution of the combination of the three drugs had a pH of 7.46-7.62, which was stable during 24 h, was without precipitation, and had a calculated osmolarity of 293 mOsm/l. High Pressure Liquid Chromatography-Mass Spectrometry found stable concentrations of fosfomycin and metronidazole both alone and in combination during 24 h. The antibacterial effect of the drug combination solution was similar to the antibacterial effects of fosfomycin and metronidazole alone.CONCLUSION: The drug combination had neutral and stable pH, was iso-osmotic, and had stable concentrations during 24 h of storage. The antibacterial effect of fosfomycin and metronidazole were not altered when the drugs were mixed.

KW - Anti-Bacterial Agents/pharmacology

KW - Drug Combinations

KW - Drug Stability

KW - Fosfomycin/pharmacology

KW - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology

KW - Humans

KW - Metronidazole/pharmacology

KW - Microbial Sensitivity Tests

KW - Recombinant Proteins/pharmacology

KW - chemistry

KW - inflammation

KW - Antibacterial drugs

U2 - 10.1055/s-0043-123933

DO - 10.1055/s-0043-123933

M3 - Journal article

C2 - 29258152

VL - 68

SP - 349

EP - 354

JO - Drug Research

JF - Drug Research

SN - 2194-9379

IS - 6

ER -

ID: 190847705