Hypothyroid women have persistently higher oxidative stress compared to healthy controls
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Hypothyroid women have persistently higher oxidative stress compared to healthy controls. / Riis, Kamilla R.; Larsen, Camilla B.; Medici, Bjarke R.; Jensen, Christian Z.; Winther, Kristian H.; Larsen, Emil L.; Ellervik, Christina; la Cour, Jeppe L.; Hegedüs, Laszlo; Brix, Thomas H.; Poulsen, Henrik E.; Knop, Filip K.; Nygaard, Birte; Bonnema, Steen J.
In: European Thyroid Journal, Vol. 12, No. 6, e230167, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Hypothyroid women have persistently higher oxidative stress compared to healthy controls
AU - Riis, Kamilla R.
AU - Larsen, Camilla B.
AU - Medici, Bjarke R.
AU - Jensen, Christian Z.
AU - Winther, Kristian H.
AU - Larsen, Emil L.
AU - Ellervik, Christina
AU - la Cour, Jeppe L.
AU - Hegedüs, Laszlo
AU - Brix, Thomas H.
AU - Poulsen, Henrik E.
AU - Knop, Filip K.
AU - Nygaard, Birte
AU - Bonnema, Steen J.
N1 - Publisher Copyright: © 2023 the author(s)
PY - 2023
Y1 - 2023
N2 - Objective: Some studies suggest that hypothyroidism is associated with increased oxidative stress. Urinary excretion of 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) represents whole-body RNA and DNA oxidation, respectively. These biomarkers have only been explored sparsely in patients with thyroid disorders. Methods: In 45 Danish women with newly diagnosed hypothyroidism, we compared 8-oxoGuo and 8-oxodG before or shortly after initiating levothyroxine with the excretion rates at euthyroidism. We also compared the excretion of 8-oxoGuo and 8-oxodG in the patients after restored euthyroidism with 18 healthy control subjects. Results: Compared with baseline, none of the biomarkers changed significantly in the patients after becoming euthyroid. The geometric mean of 8-oxoGuo was 1.63 (95% CI: 1.49–1.78) nmol/mmol creatinine at baseline and 1.67 nmol/mmol at euthyroidism (95% CI: 1.53–1.83) (P = 0.39), while that of 8-oxodG was 1.28 nmol/mmol creatinine at baseline (95% CI: 1.14–1.44) and 1.32 nmol/mmol at euthyroidism (95% CI: 1.18–1.48), respectively (P = 0.47). The relative mean differences were 0.97 (95% CI: 0.91–1.04) for 8-oxoGuo and 0.97 (95% CI: 0.88–1.06) for 8-oxodG. At baseline, multiple linear regression revealed a positive association between free thyroxine and both biomarkers (8-oxoGuo, P < 0.001; 8-oxodG, P = 0.04). Furthermore, 8-oxoGuo was positively associated with age (P = 0.04) and negatively associated with thyrotropin (P = 0.02). In the control group, the geometric mean of 8-oxoGuo was 1.23 nmol/mmol creatinine (95% CI: 1.07–1.42), while that of 8-oxodG was 1.04 nmol/mmol creatinine (95% CI: 0.88–1.23). Thus, compared with control subjects, euthyroid patients showed a significantly higher level of both 8-oxoGuo (P < 0.001) and 8-oxodG (P = 0.03). Conclusion: In hypothyroid women, no significant effect of levothyroxine treatment on the oxidative stress biomarkers 8-oxoGuo and 8-oxodG could be demonstrated. However, the excretion of these biomarkers was significantly higher than in healthy controls.
AB - Objective: Some studies suggest that hypothyroidism is associated with increased oxidative stress. Urinary excretion of 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) represents whole-body RNA and DNA oxidation, respectively. These biomarkers have only been explored sparsely in patients with thyroid disorders. Methods: In 45 Danish women with newly diagnosed hypothyroidism, we compared 8-oxoGuo and 8-oxodG before or shortly after initiating levothyroxine with the excretion rates at euthyroidism. We also compared the excretion of 8-oxoGuo and 8-oxodG in the patients after restored euthyroidism with 18 healthy control subjects. Results: Compared with baseline, none of the biomarkers changed significantly in the patients after becoming euthyroid. The geometric mean of 8-oxoGuo was 1.63 (95% CI: 1.49–1.78) nmol/mmol creatinine at baseline and 1.67 nmol/mmol at euthyroidism (95% CI: 1.53–1.83) (P = 0.39), while that of 8-oxodG was 1.28 nmol/mmol creatinine at baseline (95% CI: 1.14–1.44) and 1.32 nmol/mmol at euthyroidism (95% CI: 1.18–1.48), respectively (P = 0.47). The relative mean differences were 0.97 (95% CI: 0.91–1.04) for 8-oxoGuo and 0.97 (95% CI: 0.88–1.06) for 8-oxodG. At baseline, multiple linear regression revealed a positive association between free thyroxine and both biomarkers (8-oxoGuo, P < 0.001; 8-oxodG, P = 0.04). Furthermore, 8-oxoGuo was positively associated with age (P = 0.04) and negatively associated with thyrotropin (P = 0.02). In the control group, the geometric mean of 8-oxoGuo was 1.23 nmol/mmol creatinine (95% CI: 1.07–1.42), while that of 8-oxodG was 1.04 nmol/mmol creatinine (95% CI: 0.88–1.23). Thus, compared with control subjects, euthyroid patients showed a significantly higher level of both 8-oxoGuo (P < 0.001) and 8-oxodG (P = 0.03). Conclusion: In hypothyroid women, no significant effect of levothyroxine treatment on the oxidative stress biomarkers 8-oxoGuo and 8-oxodG could be demonstrated. However, the excretion of these biomarkers was significantly higher than in healthy controls.
KW - 8-oxo-7,8-dihydro-2′-deoxyguanosine
KW - 8-oxo-7,8-dihydroguanosine
KW - 8-oxodG
KW - 8-oxoGuo
KW - hypothyroidism
KW - oxidative stress
U2 - 10.1530/ETJ-23-0167
DO - 10.1530/ETJ-23-0167
M3 - Journal article
C2 - 37855410
AN - SCOPUS:85177170527
VL - 12
JO - European Thyroid Journal
JF - European Thyroid Journal
SN - 2235-0640
IS - 6
M1 - e230167
ER -
ID: 381558654