Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11. / Hansen, Susanne Kofoed; Borland, Helena; Hasholt, Lis Frydenreich; Tümer, Zeynep; Nielsen, Jørgen Erik; Rasmussen, Mikkel A.; Nielsen, Troels Tolstrup; Stummann, Tina C.; Fog, Karina; Hyttel, Poul.

In: Stem Cell Research, Vol. 16, No. 3, 05.2016, p. 553-556.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, SK, Borland, H, Hasholt, LF, Tümer, Z, Nielsen, JE, Rasmussen, MA, Nielsen, TT, Stummann, TC, Fog, K & Hyttel, P 2016, 'Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11', Stem Cell Research, vol. 16, no. 3, pp. 553-556. https://doi.org/10.1016/j.scr.2016.02.040

APA

Hansen, S. K., Borland, H., Hasholt, L. F., Tümer, Z., Nielsen, J. E., Rasmussen, M. A., Nielsen, T. T., Stummann, T. C., Fog, K., & Hyttel, P. (2016). Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11. Stem Cell Research, 16(3), 553-556. https://doi.org/10.1016/j.scr.2016.02.040

Vancouver

Hansen SK, Borland H, Hasholt LF, Tümer Z, Nielsen JE, Rasmussen MA et al. Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11. Stem Cell Research. 2016 May;16(3):553-556. https://doi.org/10.1016/j.scr.2016.02.040

Author

Hansen, Susanne Kofoed ; Borland, Helena ; Hasholt, Lis Frydenreich ; Tümer, Zeynep ; Nielsen, Jørgen Erik ; Rasmussen, Mikkel A. ; Nielsen, Troels Tolstrup ; Stummann, Tina C. ; Fog, Karina ; Hyttel, Poul. / Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11. In: Stem Cell Research. 2016 ; Vol. 16, No. 3. pp. 553-556.

Bibtex

@article{b246e7c12dc644a4b9bd4b2faf0b6bd1,
title = "Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11",
abstract = "Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG-repeat expanding mutation in ATXN3. We generated induced pluripotent stem cells (iPSCs) from a SCA3 patient by electroporation of dermal fibroblasts with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 and short hairpin RNA targeting P53. The resulting iPSCs had normal karyotype, were free of genomically integrated episomal plasmids, expressed pluripotency markers, could differentiate into the three germ layers in vitro and retained the disease-causing ATXN3 mutation. This iPSC line could be useful for the investigation of SCA3 disease mechanisms.",
author = "Hansen, {Susanne Kofoed} and Helena Borland and Hasholt, {Lis Frydenreich} and Zeynep T{\"u}mer and Nielsen, {J{\o}rgen Erik} and Rasmussen, {Mikkel A.} and Nielsen, {Troels Tolstrup} and Stummann, {Tina C.} and Karina Fog and Poul Hyttel",
year = "2016",
month = may,
doi = "10.1016/j.scr.2016.02.040",
language = "English",
volume = "16",
pages = "553--556",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line SCA3.A11

AU - Hansen, Susanne Kofoed

AU - Borland, Helena

AU - Hasholt, Lis Frydenreich

AU - Tümer, Zeynep

AU - Nielsen, Jørgen Erik

AU - Rasmussen, Mikkel A.

AU - Nielsen, Troels Tolstrup

AU - Stummann, Tina C.

AU - Fog, Karina

AU - Hyttel, Poul

PY - 2016/5

Y1 - 2016/5

N2 - Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG-repeat expanding mutation in ATXN3. We generated induced pluripotent stem cells (iPSCs) from a SCA3 patient by electroporation of dermal fibroblasts with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 and short hairpin RNA targeting P53. The resulting iPSCs had normal karyotype, were free of genomically integrated episomal plasmids, expressed pluripotency markers, could differentiate into the three germ layers in vitro and retained the disease-causing ATXN3 mutation. This iPSC line could be useful for the investigation of SCA3 disease mechanisms.

AB - Spinocerebellar ataxia type 3 (SCA3) is a dominantly inherited neurodegenerative disease caused by a CAG-repeat expanding mutation in ATXN3. We generated induced pluripotent stem cells (iPSCs) from a SCA3 patient by electroporation of dermal fibroblasts with episomal plasmids encoding L-MYC, LIN28, SOX2, KLF4, OCT4 and short hairpin RNA targeting P53. The resulting iPSCs had normal karyotype, were free of genomically integrated episomal plasmids, expressed pluripotency markers, could differentiate into the three germ layers in vitro and retained the disease-causing ATXN3 mutation. This iPSC line could be useful for the investigation of SCA3 disease mechanisms.

U2 - 10.1016/j.scr.2016.02.040

DO - 10.1016/j.scr.2016.02.040

M3 - Journal article

C2 - 27346190

VL - 16

SP - 553

EP - 556

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 3

ER -

ID: 163128617