TY - JOUR

T1 - In vitro recording of muscle activity induced by high intensity laser optogenetic stimulation using a diamond quantum biosensor

AU - Troise, Luca

AU - Hansen, Nikolaj Winther

AU - Olsson, Christoffer

AU - Webb, James Luke

AU - Tomasevic, Leo

AU - Achard, Jocelyn

AU - Brinza, Ovidiu

AU - Staacke, Robert

AU - Kieschnick, Michael

AU - Meijer, Jan

AU - Thielscher, Axel

AU - Siebner, Hartwig Roman

AU - Berg-Sørensen, Kirstine

AU - Perrier, Jean François

AU - Huck, Alexander

AU - Andersen, Ulrik Lund

N1 - Publisher Copyright: © 2022 Author(s).

PY - 2022

Y1 - 2022

N2 - The detection of physiological activity at the microscopic level is key for understanding the function of biosystems and relating this to their physical structure. Current sensing methods for in vitro study of living tissue often rely on invasive probes to stimulate and detect activity, bearing the risk of inducing damage in the target system. In recent years, a new type of quantum sensor based on color centers in diamond has begun to offer the possibility to instead passively sense and image living biological systems. Here, we use such a sensor to realize the recording of the biomagnetic field generated by tightly focused, high intensity pulsed laser optogenetic neuromuscular stimulation of extensor digitorum longus muscles, dissected from mice and kept alive in carbogenated solution. Recordings captured a compound action potential response and a slow signal component, which we seek to explain using a detailed model of the biological system. We show proof-of-principle experimental recording of localized neuromuscular activity from the laser stimulation site without photovoltaic or fluorescence artifacts associated with alternative techniques. Our work represents a further step toward passive sensing and imaging at the microscopic level with quantum sensing, enabling further research into mapping of neural activity and intracellular processes.

AB - The detection of physiological activity at the microscopic level is key for understanding the function of biosystems and relating this to their physical structure. Current sensing methods for in vitro study of living tissue often rely on invasive probes to stimulate and detect activity, bearing the risk of inducing damage in the target system. In recent years, a new type of quantum sensor based on color centers in diamond has begun to offer the possibility to instead passively sense and image living biological systems. Here, we use such a sensor to realize the recording of the biomagnetic field generated by tightly focused, high intensity pulsed laser optogenetic neuromuscular stimulation of extensor digitorum longus muscles, dissected from mice and kept alive in carbogenated solution. Recordings captured a compound action potential response and a slow signal component, which we seek to explain using a detailed model of the biological system. We show proof-of-principle experimental recording of localized neuromuscular activity from the laser stimulation site without photovoltaic or fluorescence artifacts associated with alternative techniques. Our work represents a further step toward passive sensing and imaging at the microscopic level with quantum sensing, enabling further research into mapping of neural activity and intracellular processes.

U2 - 10.1116/5.0106099

DO - 10.1116/5.0106099

M3 - Journal article

AN - SCOPUS:85144252395

VL - 4

JO - AVS Quantum Science

JF - AVS Quantum Science

SN - 2639-0213

IS - 4

M1 - 044402

ER -