An Evaluation of the Physicochemical Properties of Preservative-Free 0.005% (w/v) Latanoprost Ophthalmic Solutions, and the Impact on In Vitro Human Conjunctival Goblet Cell Survival
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An Evaluation of the Physicochemical Properties of Preservative-Free 0.005% (w/v) Latanoprost Ophthalmic Solutions, and the Impact on In Vitro Human Conjunctival Goblet Cell Survival. / Freiberg, Josefine C.; Hedengran, Anne; Heegaard, Steffen; Petrovski, Goran; Jacobsen, Jette; Cvenkel, Barbara; Kolko, Miriam.
In: Journal of Clinical Medicine, Vol. 11, No. 11, 3137, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - An Evaluation of the Physicochemical Properties of Preservative-Free 0.005% (w/v) Latanoprost Ophthalmic Solutions, and the Impact on In Vitro Human Conjunctival Goblet Cell Survival
AU - Freiberg, Josefine C.
AU - Hedengran, Anne
AU - Heegaard, Steffen
AU - Petrovski, Goran
AU - Jacobsen, Jette
AU - Cvenkel, Barbara
AU - Kolko, Miriam
N1 - Funding Information: Acknowledgments: Special thanks to laboratory technician Charlotte Taul for her laboratory assistance. We acknowledge the Hørslev-Fonden for their equipment grant for a tensiometer. We also acknowledge the Vissing-Fonden for their project support grant to meet the running costs. The study at the Center for Eye Research, Oslo, Norway, was partially supported by funding from the Norwegian Association of the Blind and Partially Sighted. Funding Information: Funding: This research was funded by Laboratoires Théa (France).
PY - 2022
Y1 - 2022
N2 - Purpose: To examine the physicochemical properties of five preservative-free (PF) 0.005% latanoprost ophthalmic products; Monoprost®, Latanest®, Gaap Ofteno®, Xalmono®, and Xaloptic® Free. Furthermore, the study investigated the mucin production and cell survival of primary cultured human conjunctival goblet cells when treated with PF eye drops. Method: The pH value, osmolality, and surface tension were examined. Cell survival was analyzed using lactate dehydrogenase and tetrazolium dye colorimetric assays. Mucin production was analyzed with immunohistochemical staining. Results: Monoprost® (pH value 6.84 ± 0.032) had a pH value closest to the pH value of tear fluid (pH value 7.4–7.6), whereas Gaap Ofteno® (pH value 6.34 ± 0.004) and Latanest® (pH value 6.33 ± 0.003) had the lowest pH values. Gaap Ofteno® (325.9 ± 2.9 mosmol/kg) showed iso-osmolar probabilities, whereas the other products were hypo-osmolar. Gaap Ofteno® (60.31 ± 0.35 mN/m) had a higher surface tension compared to the tear fluid (40 to 46 mN/m), as described in the literature. No significant differences in goblet cell survival or mucin release were observed between the treatments and control. Conclusion: Significant differences in pH value, osmolality, and surface tension were observed. However, this did not affect the viability of the goblet cells or the release of mucin. Clinical studies are required to evaluate the long-term effects of use on efficacy and safety.
AB - Purpose: To examine the physicochemical properties of five preservative-free (PF) 0.005% latanoprost ophthalmic products; Monoprost®, Latanest®, Gaap Ofteno®, Xalmono®, and Xaloptic® Free. Furthermore, the study investigated the mucin production and cell survival of primary cultured human conjunctival goblet cells when treated with PF eye drops. Method: The pH value, osmolality, and surface tension were examined. Cell survival was analyzed using lactate dehydrogenase and tetrazolium dye colorimetric assays. Mucin production was analyzed with immunohistochemical staining. Results: Monoprost® (pH value 6.84 ± 0.032) had a pH value closest to the pH value of tear fluid (pH value 7.4–7.6), whereas Gaap Ofteno® (pH value 6.34 ± 0.004) and Latanest® (pH value 6.33 ± 0.003) had the lowest pH values. Gaap Ofteno® (325.9 ± 2.9 mosmol/kg) showed iso-osmolar probabilities, whereas the other products were hypo-osmolar. Gaap Ofteno® (60.31 ± 0.35 mN/m) had a higher surface tension compared to the tear fluid (40 to 46 mN/m), as described in the literature. No significant differences in goblet cell survival or mucin release were observed between the treatments and control. Conclusion: Significant differences in pH value, osmolality, and surface tension were observed. However, this did not affect the viability of the goblet cells or the release of mucin. Clinical studies are required to evaluate the long-term effects of use on efficacy and safety.
KW - cell viability
KW - goblet cells
KW - latanoprost
KW - osmolality
KW - pH value
KW - preservative-free (PF)
KW - surface tension
KW - tear film
U2 - 10.3390/jcm11113137
DO - 10.3390/jcm11113137
M3 - Journal article
C2 - 35683527
AN - SCOPUS:85131077154
VL - 11
JO - Journal of Clinical Medicine
JF - Journal of Clinical Medicine
SN - 2077-0383
IS - 11
M1 - 3137
ER -
ID: 314963871