Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases

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Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases. / Kolko, M; Kiilgaard, J F; Wang, J; Poulsen, K A; Andreasen, J R; la Cour, M; Nissen, M H; Heegaard, S; Bazan, N G; Prause, J U.

In: Experimental Eye Research, Vol. 89, No. 3, 01.09.2009, p. 383-91.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kolko, M, Kiilgaard, JF, Wang, J, Poulsen, KA, Andreasen, JR, la Cour, M, Nissen, MH, Heegaard, S, Bazan, NG & Prause, JU 2009, 'Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases', Experimental Eye Research, vol. 89, no. 3, pp. 383-91. https://doi.org/10.1016/j.exer.2009.04.002

APA

Kolko, M., Kiilgaard, J. F., Wang, J., Poulsen, K. A., Andreasen, J. R., la Cour, M., Nissen, M. H., Heegaard, S., Bazan, N. G., & Prause, J. U. (2009). Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases. Experimental Eye Research, 89(3), 383-91. https://doi.org/10.1016/j.exer.2009.04.002

Vancouver

Kolko M, Kiilgaard JF, Wang J, Poulsen KA, Andreasen JR, la Cour M et al. Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases. Experimental Eye Research. 2009 Sep 1;89(3):383-91. https://doi.org/10.1016/j.exer.2009.04.002

Author

Kolko, M ; Kiilgaard, J F ; Wang, J ; Poulsen, K A ; Andreasen, J R ; la Cour, M ; Nissen, M H ; Heegaard, S ; Bazan, N G ; Prause, J U. / Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases. In: Experimental Eye Research. 2009 ; Vol. 89, No. 3. pp. 383-91.

Bibtex

@article{69cc634067dd11df928f000ea68e967b,
title = "Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases",
abstract = "Calcium-independent phospholipase A2, group VIA (iPLA2-VIA) is involved in cell proliferation. This study aimed to evaluate the role of iPLA2-VIA in retinal pigment epithelium (RPE) cell proliferation and in retinal diseases involving RPE proliferation. A human RPE cell line (ARPE-19) was used to explore this role in vitro. Proliferating ARPE-19 cells had increased expression and activity of iPLA2-VIA. iPLA2-VIA was found in the nuclei of proliferating ARPE-19 cells, whereas in confluent ARPE-19 cells, with limited proliferation, iPLA2-VIA was primarily found in the cytosol. Inhibition of iPLA2-VIA decreased the rate of proliferation, whereas over expression of iPLA2-VIA increased the rate of proliferation. Using an experimental porcine model of RPE proliferation we demonstrated significant nuclear upregulation of iPLA2-VIA in proliferating RPE cells in vivo. We furthermore evaluated the expression of iPLA2-VIA in proliferative vitreoretinopathy (PVR). PVR membranes revealed nuclear expression of iPLA2-VIA in the RPE cells which had migrated and participated in the formation of the membranes. Overall, the present results point to an important role of iPLA2-VIA in the regulation of RPE proliferation suggesting that iPLA2-VIA may be considered as a possible pharmaceutical target in retinal diseases involving RPE proliferation and migration.",
author = "M Kolko and Kiilgaard, {J F} and J Wang and Poulsen, {K A} and Andreasen, {J R} and {la Cour}, M and Nissen, {M H} and S Heegaard and Bazan, {N G} and Prause, {J U}",
year = "2009",
month = sep,
day = "1",
doi = "10.1016/j.exer.2009.04.002",
language = "English",
volume = "89",
pages = "383--91",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press",
number = "3",

}

RIS

TY - JOUR

T1 - Calcium-independent phospholipase A2 regulates retinal pigment epithelium proliferation and may be important in the pathogenesis of retinal diseases

AU - Kolko, M

AU - Kiilgaard, J F

AU - Wang, J

AU - Poulsen, K A

AU - Andreasen, J R

AU - la Cour, M

AU - Nissen, M H

AU - Heegaard, S

AU - Bazan, N G

AU - Prause, J U

PY - 2009/9/1

Y1 - 2009/9/1

N2 - Calcium-independent phospholipase A2, group VIA (iPLA2-VIA) is involved in cell proliferation. This study aimed to evaluate the role of iPLA2-VIA in retinal pigment epithelium (RPE) cell proliferation and in retinal diseases involving RPE proliferation. A human RPE cell line (ARPE-19) was used to explore this role in vitro. Proliferating ARPE-19 cells had increased expression and activity of iPLA2-VIA. iPLA2-VIA was found in the nuclei of proliferating ARPE-19 cells, whereas in confluent ARPE-19 cells, with limited proliferation, iPLA2-VIA was primarily found in the cytosol. Inhibition of iPLA2-VIA decreased the rate of proliferation, whereas over expression of iPLA2-VIA increased the rate of proliferation. Using an experimental porcine model of RPE proliferation we demonstrated significant nuclear upregulation of iPLA2-VIA in proliferating RPE cells in vivo. We furthermore evaluated the expression of iPLA2-VIA in proliferative vitreoretinopathy (PVR). PVR membranes revealed nuclear expression of iPLA2-VIA in the RPE cells which had migrated and participated in the formation of the membranes. Overall, the present results point to an important role of iPLA2-VIA in the regulation of RPE proliferation suggesting that iPLA2-VIA may be considered as a possible pharmaceutical target in retinal diseases involving RPE proliferation and migration.

AB - Calcium-independent phospholipase A2, group VIA (iPLA2-VIA) is involved in cell proliferation. This study aimed to evaluate the role of iPLA2-VIA in retinal pigment epithelium (RPE) cell proliferation and in retinal diseases involving RPE proliferation. A human RPE cell line (ARPE-19) was used to explore this role in vitro. Proliferating ARPE-19 cells had increased expression and activity of iPLA2-VIA. iPLA2-VIA was found in the nuclei of proliferating ARPE-19 cells, whereas in confluent ARPE-19 cells, with limited proliferation, iPLA2-VIA was primarily found in the cytosol. Inhibition of iPLA2-VIA decreased the rate of proliferation, whereas over expression of iPLA2-VIA increased the rate of proliferation. Using an experimental porcine model of RPE proliferation we demonstrated significant nuclear upregulation of iPLA2-VIA in proliferating RPE cells in vivo. We furthermore evaluated the expression of iPLA2-VIA in proliferative vitreoretinopathy (PVR). PVR membranes revealed nuclear expression of iPLA2-VIA in the RPE cells which had migrated and participated in the formation of the membranes. Overall, the present results point to an important role of iPLA2-VIA in the regulation of RPE proliferation suggesting that iPLA2-VIA may be considered as a possible pharmaceutical target in retinal diseases involving RPE proliferation and migration.

U2 - 10.1016/j.exer.2009.04.002

DO - 10.1016/j.exer.2009.04.002

M3 - Journal article

C2 - 19379734

VL - 89

SP - 383

EP - 391

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 3

ER -

ID: 19952403