Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain
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Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain. / Kolko, Miriam; Christoffersen, Nanna Rønbjerg; Barreiro, S.G.; Miller, M.L.; Pizza, A.J.; Bazan, N.G.
In: Journal of Neuroscience Research, Vol. 83, No. 5, 2006, p. 874-882.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization and location of secretory phospholipase A2 groups IIE, V, and X in the rat brain
AU - Kolko, Miriam
AU - Christoffersen, Nanna Rønbjerg
AU - Barreiro, S.G.
AU - Miller, M.L.
AU - Pizza, A.J.
AU - Bazan, N.G.
PY - 2006
Y1 - 2006
N2 - Secretory phospholipases A2 (sPLA2) form a diverse family of enzymes involved in a variety of physiologic and pathologic processes. Common among all sPLA2 is the ability to cleave the second position of phospholipids, thereby releasing fatty acid and a lysophospholipid. Several sPLA2 have been cloned and characterized in various tissues and receptors have been identified. In the nervous system, sPLA2 groups IB, IIA, IIE, IIF, V, and XII have been identified, and binding sites for sPLA2 have been found. Here, we report sPLA2-IIE and sPLA2-X in rat brain as well as in neurons in primary culture. We furthermore confirm the presence of sPLA2-V in rat brain and demonstrate the presence of sPLA2-V in primary neuronal cultures. The distribution of sPLA2-IIE, V, and -X seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. We also find that sPLA2-IIE, -V, and -X are differentially induced by kainic acid. This study supports the concept that sPLA2 heterogeneity in brain is functionally relevant and responsive to seizures.
AB - Secretory phospholipases A2 (sPLA2) form a diverse family of enzymes involved in a variety of physiologic and pathologic processes. Common among all sPLA2 is the ability to cleave the second position of phospholipids, thereby releasing fatty acid and a lysophospholipid. Several sPLA2 have been cloned and characterized in various tissues and receptors have been identified. In the nervous system, sPLA2 groups IB, IIA, IIE, IIF, V, and XII have been identified, and binding sites for sPLA2 have been found. Here, we report sPLA2-IIE and sPLA2-X in rat brain as well as in neurons in primary culture. We furthermore confirm the presence of sPLA2-V in rat brain and demonstrate the presence of sPLA2-V in primary neuronal cultures. The distribution of sPLA2-IIE, V, and -X seems to be mainly neuronal, with the highest abundance occurring in the cerebral cortex and hippocampus. We also find that sPLA2-IIE, -V, and -X are differentially induced by kainic acid. This study supports the concept that sPLA2 heterogeneity in brain is functionally relevant and responsive to seizures.
KW - Animals
KW - Brain
KW - Cells, Cultured
KW - Embryo, Mammalian
KW - Group II Phospholipases A2
KW - Immunohistochemistry
KW - In Situ Hybridization
KW - Neurons
KW - Phospholipases A
KW - Phospholipases A2
KW - Rats
KW - Reverse Transcriptase Polymerase Chain Reaction
U2 - 10.1002/jnr.20773
DO - 10.1002/jnr.20773
M3 - Journal article
C2 - 16511882
VL - 83
SP - 874
EP - 882
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 5
ER -
ID: 1203153