Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB. / Zhan, Chen; Wang, Jinmei; Kolko, Miriam.

In: Current Eye Research, Vol. 37, No. 10, 10.2012, p. 930-940.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhan, C, Wang, J & Kolko, M 2012, 'Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB', Current Eye Research, vol. 37, no. 10, pp. 930-940. https://doi.org/10.3109/02713683.2012.691598

APA

Zhan, C., Wang, J., & Kolko, M. (2012). Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB. Current Eye Research, 37(10), 930-940. https://doi.org/10.3109/02713683.2012.691598

Vancouver

Zhan C, Wang J, Kolko M. Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB. Current Eye Research. 2012 Oct;37(10):930-940. https://doi.org/10.3109/02713683.2012.691598

Author

Zhan, Chen ; Wang, Jinmei ; Kolko, Miriam. / Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB. In: Current Eye Research. 2012 ; Vol. 37, No. 10. pp. 930-940.

Bibtex

@article{4b4709d9ca17461eacf04592b7c19edc,
title = "Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB",
abstract = "PURPOSE: To investigate the roles of the phospholipases A(2) (PLA(2)) subtypes, iPLA(2)-VIA and sPLA(2)-IB in retinal pigment epithelium (RPE) phagocytosis of photoreceptor outer segments (POS) and to explore a possible interaction between sPLA(2)-IB and iPLA(2)-VIA in the RPE.METHODS: To explore the role of iPLA(2)-VIA in RPE phagocytosis of POS, experiments with iPLA(2)-VIA vector transfection, iPLA(2)-VIA(-/-) knockout (KO) mice, and iPLA(2)-VIA inhibition by bromoenol lactone (BEL) were done. Exogenous addition of sPLA(2)-IB was used to investigate the role of sPLA(2)-IB in RPE phagocytosis. A Luciferase Reporter Vector containing the iPLA(2)-VIA promoter was used to study the effects of sPLA(2)-IB on the iPLA(2)-VIA promoter.RESULTS: ARPE-19 and primary mouse RPE cells transfected with iPLA(2)-VIA showed increased phagocytosis. Phagocytosis was reduced in primary mouse RPE inhibited with BEL and in RPE from KO mice. Exogenous addition of enzymatically active and inactive sPLA(2)-IB reduced phagocytosis in ARPE-19 and primary mouse RPE cells. Finally, sPLA(2)-IB did not seem to affect the iPLA(2)-VIA promoter.CONCLUSION: The present study confirms the involvement of iPLA(2)-VIA in efficient RPE phagocytosis of POS, while exogenously added sPLA(2)-IB decreases phagocytosis regardless of enzymatic activity. No apparent interaction between iPLA(2)-VIA and sPLA(2)-IB was found.",
keywords = "Animals, Cattle, Cell Line, Cell Survival, Gene Expression Regulation, Enzymologic, Genes, Reporter, Group IB Phospholipases A2, Group VI Phospholipases A2, Humans, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Phagocytosis, Primary Cell Culture, Promoter Regions, Genetic, RNA, Messenger, Retinal Photoreceptor Cell Outer Segment, Retinal Pigment Epithelium",
author = "Chen Zhan and Jinmei Wang and Miriam Kolko",
year = "2012",
month = oct,
doi = "10.3109/02713683.2012.691598",
language = "English",
volume = "37",
pages = "930--940",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Taylor & Francis",
number = "10",

}

RIS

TY - JOUR

T1 - Diverse regulation of retinal pigment epithelium phagocytosis of photoreceptor outer segments by calcium-independent phospholipase A₂, group VIA and secretory phospholipase A₂, group IB

AU - Zhan, Chen

AU - Wang, Jinmei

AU - Kolko, Miriam

PY - 2012/10

Y1 - 2012/10

N2 - PURPOSE: To investigate the roles of the phospholipases A(2) (PLA(2)) subtypes, iPLA(2)-VIA and sPLA(2)-IB in retinal pigment epithelium (RPE) phagocytosis of photoreceptor outer segments (POS) and to explore a possible interaction between sPLA(2)-IB and iPLA(2)-VIA in the RPE.METHODS: To explore the role of iPLA(2)-VIA in RPE phagocytosis of POS, experiments with iPLA(2)-VIA vector transfection, iPLA(2)-VIA(-/-) knockout (KO) mice, and iPLA(2)-VIA inhibition by bromoenol lactone (BEL) were done. Exogenous addition of sPLA(2)-IB was used to investigate the role of sPLA(2)-IB in RPE phagocytosis. A Luciferase Reporter Vector containing the iPLA(2)-VIA promoter was used to study the effects of sPLA(2)-IB on the iPLA(2)-VIA promoter.RESULTS: ARPE-19 and primary mouse RPE cells transfected with iPLA(2)-VIA showed increased phagocytosis. Phagocytosis was reduced in primary mouse RPE inhibited with BEL and in RPE from KO mice. Exogenous addition of enzymatically active and inactive sPLA(2)-IB reduced phagocytosis in ARPE-19 and primary mouse RPE cells. Finally, sPLA(2)-IB did not seem to affect the iPLA(2)-VIA promoter.CONCLUSION: The present study confirms the involvement of iPLA(2)-VIA in efficient RPE phagocytosis of POS, while exogenously added sPLA(2)-IB decreases phagocytosis regardless of enzymatic activity. No apparent interaction between iPLA(2)-VIA and sPLA(2)-IB was found.

AB - PURPOSE: To investigate the roles of the phospholipases A(2) (PLA(2)) subtypes, iPLA(2)-VIA and sPLA(2)-IB in retinal pigment epithelium (RPE) phagocytosis of photoreceptor outer segments (POS) and to explore a possible interaction between sPLA(2)-IB and iPLA(2)-VIA in the RPE.METHODS: To explore the role of iPLA(2)-VIA in RPE phagocytosis of POS, experiments with iPLA(2)-VIA vector transfection, iPLA(2)-VIA(-/-) knockout (KO) mice, and iPLA(2)-VIA inhibition by bromoenol lactone (BEL) were done. Exogenous addition of sPLA(2)-IB was used to investigate the role of sPLA(2)-IB in RPE phagocytosis. A Luciferase Reporter Vector containing the iPLA(2)-VIA promoter was used to study the effects of sPLA(2)-IB on the iPLA(2)-VIA promoter.RESULTS: ARPE-19 and primary mouse RPE cells transfected with iPLA(2)-VIA showed increased phagocytosis. Phagocytosis was reduced in primary mouse RPE inhibited with BEL and in RPE from KO mice. Exogenous addition of enzymatically active and inactive sPLA(2)-IB reduced phagocytosis in ARPE-19 and primary mouse RPE cells. Finally, sPLA(2)-IB did not seem to affect the iPLA(2)-VIA promoter.CONCLUSION: The present study confirms the involvement of iPLA(2)-VIA in efficient RPE phagocytosis of POS, while exogenously added sPLA(2)-IB decreases phagocytosis regardless of enzymatic activity. No apparent interaction between iPLA(2)-VIA and sPLA(2)-IB was found.

KW - Animals

KW - Cattle

KW - Cell Line

KW - Cell Survival

KW - Gene Expression Regulation, Enzymologic

KW - Genes, Reporter

KW - Group IB Phospholipases A2

KW - Group VI Phospholipases A2

KW - Humans

KW - Mice

KW - Mice, 129 Strain

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Phagocytosis

KW - Primary Cell Culture

KW - Promoter Regions, Genetic

KW - RNA, Messenger

KW - Retinal Photoreceptor Cell Outer Segment

KW - Retinal Pigment Epithelium

U2 - 10.3109/02713683.2012.691598

DO - 10.3109/02713683.2012.691598

M3 - Journal article

C2 - 22680611

VL - 37

SP - 930

EP - 940

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 10

ER -

ID: 128614601