Non-invasive tryptophan fluorescence measurements as a novel method of grading cataract
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Non-invasive tryptophan fluorescence measurements as a novel method of grading cataract. / Erichsen, Jesper Høiberg; Mensah, Aurore; Kessel, Line.
In: Experimental Eye Research, Vol. 165, 12.2017, p. 59-64.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Non-invasive tryptophan fluorescence measurements as a novel method of grading cataract
AU - Erichsen, Jesper Høiberg
AU - Mensah, Aurore
AU - Kessel, Line
PY - 2017/12
Y1 - 2017/12
N2 - Development of non-invasive treatments for cataract calls for a sensitive diagnostic assay. We conducted a study to test whether the ratio of folded tryptophan to non-tryptophan fluorescence emission (F-factor) may be used for grading cataracts in human lenses. The F-factor was measured on aspirated lens material from eyes undergoing femtosecond laser assisted cataract surgery (FLACS) and was compared to a preoperative optical grading of cataract using Scheimpflug imaging. The preoperative optical grading allocated the cataracts to 1 of 4 categories according to the density of the cataract. All cataracts were age-related. Lens material from 16 eyes of 14 patients was included in the study. Cataracts were preoperatively graded in categories 1, 2 and 3. No lenses were category 4. For nuclear cataracts mean values of F-factor were 52.9 (SD 12.2), 61.7 (SD 5.3) and 75.7 (SD 8.9) for categories 1, 2 and 3 respectively. Linear regression on F-factor as a function of preoperative grading category showed increasing values of F-factor with increasing preoperative grading category, R2 = 0.515. Our experiment showed that preoperative optical grading of cataracts by Scheimpflug imaging may correlate to measures of tryptophan and non-tryptophan fluorescence in human lenses. Based on our results we find that measuring the ratio between tryptophan- and non-tryptophan fluorescence may be a future tool for grading cataracts, but further research is needed.
AB - Development of non-invasive treatments for cataract calls for a sensitive diagnostic assay. We conducted a study to test whether the ratio of folded tryptophan to non-tryptophan fluorescence emission (F-factor) may be used for grading cataracts in human lenses. The F-factor was measured on aspirated lens material from eyes undergoing femtosecond laser assisted cataract surgery (FLACS) and was compared to a preoperative optical grading of cataract using Scheimpflug imaging. The preoperative optical grading allocated the cataracts to 1 of 4 categories according to the density of the cataract. All cataracts were age-related. Lens material from 16 eyes of 14 patients was included in the study. Cataracts were preoperatively graded in categories 1, 2 and 3. No lenses were category 4. For nuclear cataracts mean values of F-factor were 52.9 (SD 12.2), 61.7 (SD 5.3) and 75.7 (SD 8.9) for categories 1, 2 and 3 respectively. Linear regression on F-factor as a function of preoperative grading category showed increasing values of F-factor with increasing preoperative grading category, R2 = 0.515. Our experiment showed that preoperative optical grading of cataracts by Scheimpflug imaging may correlate to measures of tryptophan and non-tryptophan fluorescence in human lenses. Based on our results we find that measuring the ratio between tryptophan- and non-tryptophan fluorescence may be a future tool for grading cataracts, but further research is needed.
KW - Cataract
KW - Fluorescence
KW - Grading
KW - Non-invasive
KW - Scheimpflug
KW - Tryptophan
U2 - 10.1016/j.exer.2017.09.006
DO - 10.1016/j.exer.2017.09.006
M3 - Journal article
C2 - 28935513
AN - SCOPUS:85029808209
VL - 165
SP - 59
EP - 64
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
ER -
ID: 196710789