Secretory phospholipase A2 potentiates glutamate-induced rat striatal neuronal cell death in vivo
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Secretory phospholipase A2 potentiates glutamate-induced rat striatal neuronal cell death in vivo. / Kolko, M; Bruhn, T; Christensen, Thomas; Lazdunski, M; Lambeau, G; Bazan, N G; Diemer, Nils Henrik.
In: Neuroscience Letters, Vol. 274, No. 3, 1999, p. 167-170.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Secretory phospholipase A2 potentiates glutamate-induced rat striatal neuronal cell death in vivo
AU - Kolko, M
AU - Bruhn, T
AU - Christensen, Thomas
AU - Lazdunski, M
AU - Lambeau, G
AU - Bazan, N G
AU - Diemer, Nils Henrik
PY - 1999
Y1 - 1999
N2 - The secretory phospholipases A2 (sPLA2) OS2 (10, 20 and 50 pmol) or OS1, (50 pmol) purified from taipan snake Oxyuranus scutellatus scutellatus venom, and the excitatory amino acid glutamate (Glu) (2.5 and 5.0 micromol) were injected into the right striatum of male Wistar rats. Injection of 10 and 20 pmol OS2 caused no neurological abnormalities or tissue damage. OS2 (50 pmol) caused apathy and circling towards the injection side. Histology revealed an infarct at the injection site. Injection of 50 pmol OS1 showed very little or no signs of neurotoxicity. Injection of 2.5 micromol Glu caused no tissue damage or neurological abnormality. After injection of 5.0 micromol Glu, the animals initially circled towards the side of injection, and gradually developed generalized clonic convulsions. These animals showed a well demarcated striatal infarct. When non-toxic concentrations of 20 pmol OS2 and 2.5 micromol Glu were co-injected, a synergistic neurotoxicity was observed. Extensive histological damage occurred in the entire right hemisphere, and in several rats comprising part of the contralateral hemisphere. These animals were apathetic in the immediate hours following injection, with circling towards the side of injection in the following days. Thus, OS2 greatly potentiates glutamate excitoxicity in vivo.
AB - The secretory phospholipases A2 (sPLA2) OS2 (10, 20 and 50 pmol) or OS1, (50 pmol) purified from taipan snake Oxyuranus scutellatus scutellatus venom, and the excitatory amino acid glutamate (Glu) (2.5 and 5.0 micromol) were injected into the right striatum of male Wistar rats. Injection of 10 and 20 pmol OS2 caused no neurological abnormalities or tissue damage. OS2 (50 pmol) caused apathy and circling towards the injection side. Histology revealed an infarct at the injection site. Injection of 50 pmol OS1 showed very little or no signs of neurotoxicity. Injection of 2.5 micromol Glu caused no tissue damage or neurological abnormality. After injection of 5.0 micromol Glu, the animals initially circled towards the side of injection, and gradually developed generalized clonic convulsions. These animals showed a well demarcated striatal infarct. When non-toxic concentrations of 20 pmol OS2 and 2.5 micromol Glu were co-injected, a synergistic neurotoxicity was observed. Extensive histological damage occurred in the entire right hemisphere, and in several rats comprising part of the contralateral hemisphere. These animals were apathetic in the immediate hours following injection, with circling towards the side of injection in the following days. Thus, OS2 greatly potentiates glutamate excitoxicity in vivo.
KW - Animals
KW - Behavior, Animal
KW - Cell Death
KW - Cerebral Infarction
KW - Corpus Striatum
KW - Elapid Venoms
KW - Glutamic Acid
KW - Male
KW - Microinjections
KW - Neurons
KW - Neurotoxins
KW - Phospholipases A
KW - Phospholipases A2
KW - Rats
KW - Rats, Wistar
KW - Reptilian Proteins
U2 - 10.1016/S0304-3940(99)00709-0
DO - 10.1016/S0304-3940(99)00709-0
M3 - Journal article
C2 - 10548416
VL - 274
SP - 167
EP - 170
JO - Neuroscience letters. Supplement
JF - Neuroscience letters. Supplement
SN - 0167-6253
IS - 3
ER -
ID: 197763