Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling

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Standard

Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling. / Almstrup, Kristian; Hoei-Hansen, Christina E.; Wirkner, Ute; Blake, Jonathon; Schwager, Christian; Ansorge, Wilhelm; Nielsen, John E.; Skakkebæk, Niels E.; Rajpert-De Meyts, Ewa; Leffers, Henrik.

In: Cancer Research, Vol. 64, No. 14, 15.07.2004, p. 4736-4743.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Almstrup, K, Hoei-Hansen, CE, Wirkner, U, Blake, J, Schwager, C, Ansorge, W, Nielsen, JE, Skakkebæk, NE, Rajpert-De Meyts, E & Leffers, H 2004, 'Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling', Cancer Research, vol. 64, no. 14, pp. 4736-4743. https://doi.org/10.1158/0008-5472.CAN-04-0679

APA

Almstrup, K., Hoei-Hansen, C. E., Wirkner, U., Blake, J., Schwager, C., Ansorge, W., Nielsen, J. E., Skakkebæk, N. E., Rajpert-De Meyts, E., & Leffers, H. (2004). Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling. Cancer Research, 64(14), 4736-4743. https://doi.org/10.1158/0008-5472.CAN-04-0679

Vancouver

Almstrup K, Hoei-Hansen CE, Wirkner U, Blake J, Schwager C, Ansorge W et al. Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling. Cancer Research. 2004 Jul 15;64(14):4736-4743. https://doi.org/10.1158/0008-5472.CAN-04-0679

Author

Almstrup, Kristian ; Hoei-Hansen, Christina E. ; Wirkner, Ute ; Blake, Jonathon ; Schwager, Christian ; Ansorge, Wilhelm ; Nielsen, John E. ; Skakkebæk, Niels E. ; Rajpert-De Meyts, Ewa ; Leffers, Henrik. / Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling. In: Cancer Research. 2004 ; Vol. 64, No. 14. pp. 4736-4743.

Bibtex

@article{1d6d5161de614b03b1792bb70e2fde21,
title = "Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling",
abstract = "Carcinoma in situ (CIS) is the common precursor of histologically heterogeneous testicular germ cell tumors (TGCTs), which in recent decades have markedly increased and now are the most common malignancy of young men. Using genome-wide gene expression profiling, we identified >200 genes highly expressed in testicular CIS, including many never reported in testicular neoplasms. Expression was further verified by semiquantitative reverse transcription-PCR and in situ hybridization. Among the highest expressed genes were NANOG and POU5F1, and reverse transcription-PCR revealed possible changes in their stoichiometry on progression into embryonic carcinoma. We compared the CIS expression profile with patterns reported in embryonic stem cells (ESCs), which revealed a substantial overlap that may be as high as 50%. We also demonstrated an over-representation of expressed genes in regions of 17q and 12, reported as unstable in cultured ESCs. The close similarity between CIS and ESCs explains the pluripotency of CIS. Moreover, the findings are consistent with an early prenatal origin of TGCTs and thus suggest that etiologic factors operating in utero are of primary importance for the incidence trends of TGCTs. Finally, some of the highly expressed genes identified in this study are promising candidates for new diagnostic markers for CIS and/or TGCTs.",
author = "Kristian Almstrup and Hoei-Hansen, {Christina E.} and Ute Wirkner and Jonathon Blake and Christian Schwager and Wilhelm Ansorge and Nielsen, {John E.} and Skakkeb{\ae}k, {Niels E.} and {Rajpert-De Meyts}, Ewa and Henrik Leffers",
year = "2004",
month = jul,
day = "15",
doi = "10.1158/0008-5472.CAN-04-0679",
language = "English",
volume = "64",
pages = "4736--4743",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research",
number = "14",

}

RIS

TY - JOUR

T1 - Embryonic stem cell-like features of testicular carcinoma in situ revealed by genome-wide gene expression profiling

AU - Almstrup, Kristian

AU - Hoei-Hansen, Christina E.

AU - Wirkner, Ute

AU - Blake, Jonathon

AU - Schwager, Christian

AU - Ansorge, Wilhelm

AU - Nielsen, John E.

AU - Skakkebæk, Niels E.

AU - Rajpert-De Meyts, Ewa

AU - Leffers, Henrik

PY - 2004/7/15

Y1 - 2004/7/15

N2 - Carcinoma in situ (CIS) is the common precursor of histologically heterogeneous testicular germ cell tumors (TGCTs), which in recent decades have markedly increased and now are the most common malignancy of young men. Using genome-wide gene expression profiling, we identified >200 genes highly expressed in testicular CIS, including many never reported in testicular neoplasms. Expression was further verified by semiquantitative reverse transcription-PCR and in situ hybridization. Among the highest expressed genes were NANOG and POU5F1, and reverse transcription-PCR revealed possible changes in their stoichiometry on progression into embryonic carcinoma. We compared the CIS expression profile with patterns reported in embryonic stem cells (ESCs), which revealed a substantial overlap that may be as high as 50%. We also demonstrated an over-representation of expressed genes in regions of 17q and 12, reported as unstable in cultured ESCs. The close similarity between CIS and ESCs explains the pluripotency of CIS. Moreover, the findings are consistent with an early prenatal origin of TGCTs and thus suggest that etiologic factors operating in utero are of primary importance for the incidence trends of TGCTs. Finally, some of the highly expressed genes identified in this study are promising candidates for new diagnostic markers for CIS and/or TGCTs.

AB - Carcinoma in situ (CIS) is the common precursor of histologically heterogeneous testicular germ cell tumors (TGCTs), which in recent decades have markedly increased and now are the most common malignancy of young men. Using genome-wide gene expression profiling, we identified >200 genes highly expressed in testicular CIS, including many never reported in testicular neoplasms. Expression was further verified by semiquantitative reverse transcription-PCR and in situ hybridization. Among the highest expressed genes were NANOG and POU5F1, and reverse transcription-PCR revealed possible changes in their stoichiometry on progression into embryonic carcinoma. We compared the CIS expression profile with patterns reported in embryonic stem cells (ESCs), which revealed a substantial overlap that may be as high as 50%. We also demonstrated an over-representation of expressed genes in regions of 17q and 12, reported as unstable in cultured ESCs. The close similarity between CIS and ESCs explains the pluripotency of CIS. Moreover, the findings are consistent with an early prenatal origin of TGCTs and thus suggest that etiologic factors operating in utero are of primary importance for the incidence trends of TGCTs. Finally, some of the highly expressed genes identified in this study are promising candidates for new diagnostic markers for CIS and/or TGCTs.

UR - http://www.scopus.com/inward/record.url?scp=3142779194&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-04-0679

DO - 10.1158/0008-5472.CAN-04-0679

M3 - Journal article

C2 - 15256440

AN - SCOPUS:3142779194

VL - 64

SP - 4736

EP - 4743

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 14

ER -

ID: 284208303