Fluispotter, a novel automated and wearable device for accurate volume serial dried blood spot sampling
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- bio-2020-0048
Final published version, 2.73 MB, PDF document
Aim: A novel automated serial dried blood spot (DBS) sampler, 'Fluispotter', was tested for its sampling performance. Materials & methods: An LC-MS/MS method was developed for the analysis of cortisol in DBS samples serially spotted by Fluispotter. The cortisol concentrations in 148 paired DBS and plasma samples were compared across a hematocrit (HCT) range of 22-55%. Results: The interassay accuracy and precision were <10%. Overall assay bias was negligible across the HCTs tested when analyzing the whole-spot DBS samples. The accuracy and precision of the blood volume in 10 μl DBS samples spotted by Fluispotters and micropipettes were within 3%. Deming regression and Bland-Altman analysis showed a good agreement of DBS-predicted and measured plasma cortisol. Conclusion: The Fluispotter performed serial sampling with high accuracy and precision of the sample blood volume.
Original language | English |
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Journal | Bioanalysis |
Volume | 12 |
Issue number | 10 |
Pages (from-to) | 665-681 |
Number of pages | 17 |
ISSN | 1757-6180 |
DOIs | |
Publication status | Published - May 2020 |
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