Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours

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Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours. / Almstrup, K.; Hoei-Hansen, C. E.; Nielsen, J. E.; Wirkner, U.; Ansorge, W.; Skakkebæk, N. E.; Rajpert-De Meyts, E.; Leffers, H.

In: British Journal of Cancer, Vol. 92, No. 10, 23.05.2005, p. 1934-1941.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Almstrup, K, Hoei-Hansen, CE, Nielsen, JE, Wirkner, U, Ansorge, W, Skakkebæk, NE, Rajpert-De Meyts, E & Leffers, H 2005, 'Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours', British Journal of Cancer, vol. 92, no. 10, pp. 1934-1941. https://doi.org/10.1038/sj.bjc.6602560

APA

Almstrup, K., Hoei-Hansen, C. E., Nielsen, J. E., Wirkner, U., Ansorge, W., Skakkebæk, N. E., Rajpert-De Meyts, E., & Leffers, H. (2005). Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours. British Journal of Cancer, 92(10), 1934-1941. https://doi.org/10.1038/sj.bjc.6602560

Vancouver

Almstrup K, Hoei-Hansen CE, Nielsen JE, Wirkner U, Ansorge W, Skakkebæk NE et al. Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours. British Journal of Cancer. 2005 May 23;92(10):1934-1941. https://doi.org/10.1038/sj.bjc.6602560

Author

Almstrup, K. ; Hoei-Hansen, C. E. ; Nielsen, J. E. ; Wirkner, U. ; Ansorge, W. ; Skakkebæk, N. E. ; Rajpert-De Meyts, E. ; Leffers, H. / Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours. In: British Journal of Cancer. 2005 ; Vol. 92, No. 10. pp. 1934-1941.

Bibtex

@article{19b5b8bf92f04f9a9172adb8733e6f8a,
title = "Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours",
abstract = "The carcinoma in situ (CIS) cell is the common precursor of nearly all testicular germ cell tumours (TGCT). In a previous study, we examined the gene expression profile of CIS cells and found many features common to embryonic stem cells indicating that initiation of neoplastic transformation into CIS occurs early during foetal life. Progression into an overt tumour, however, typically first happens after puberty, where CIS cells transform into either a seminoma (SEM) or a nonseminoma (N-SEM). Here, we have compared the genome-wide gene expression of CIS cells to that of testicular SEM and a sample containing a mixture of N-SEM components, and analyse the data together with the previously published data on CIS. Genes showing expression in the SEM or N-SEM were selected, in order to identify gene expression markers associated with the progression of CIS cells. The identified markers were verified by reverse transcriptase-polymerase chain reaction and in situ hybridisation in a range of different TGCT samples. Verification showed some interpatient variation, but combined analysis of a range of the identified markers may discriminate TGCT samples as SEMs or N-SEMs. Of particular interest, we found that both DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta) and DNMT3L (DNA (cytosine-5-)-methyltransferase 3 like) were overexpressed in the N-SEMs, indicating the epigenetic differences between N-SEMs and classical SEM.",
keywords = "Carcinoma in situ, DNMT3L, Gene expression, Nonseminoma, Seminoma, Testicular cancer",
author = "K. Almstrup and Hoei-Hansen, {C. E.} and Nielsen, {J. E.} and U. Wirkner and W. Ansorge and Skakkeb{\ae}k, {N. E.} and {Rajpert-De Meyts}, E. and H. Leffers",
note = "Funding Information: We would like to thank Marlene Dalgaard, Brian Vendelbo and Sabina Soultanova for excellent technical assistance, and Dr Peter W Andrews for the kind gift of tumour cell lines. This work was supported by grants from the Danish Cancer Society, Svend Andersen{\textquoteright}s foundation, the Danish Medical Research Council and the European Union. We are solely responsible for statements made and the article does not represent the opinion of The European Commission, which is not responsible for any use that might be made of data appearing therein.",
year = "2005",
month = may,
day = "23",
doi = "10.1038/sj.bjc.6602560",
language = "English",
volume = "92",
pages = "1934--1941",
journal = "The British journal of cancer. Supplement",
issn = "0007-0920",
publisher = "nature publishing group",
number = "10",

}

RIS

TY - JOUR

T1 - Genome-wide gene expression profiling of testicular carcinoma in situ progression into overt tumours

AU - Almstrup, K.

AU - Hoei-Hansen, C. E.

AU - Nielsen, J. E.

AU - Wirkner, U.

AU - Ansorge, W.

AU - Skakkebæk, N. E.

AU - Rajpert-De Meyts, E.

AU - Leffers, H.

N1 - Funding Information: We would like to thank Marlene Dalgaard, Brian Vendelbo and Sabina Soultanova for excellent technical assistance, and Dr Peter W Andrews for the kind gift of tumour cell lines. This work was supported by grants from the Danish Cancer Society, Svend Andersen’s foundation, the Danish Medical Research Council and the European Union. We are solely responsible for statements made and the article does not represent the opinion of The European Commission, which is not responsible for any use that might be made of data appearing therein.

PY - 2005/5/23

Y1 - 2005/5/23

N2 - The carcinoma in situ (CIS) cell is the common precursor of nearly all testicular germ cell tumours (TGCT). In a previous study, we examined the gene expression profile of CIS cells and found many features common to embryonic stem cells indicating that initiation of neoplastic transformation into CIS occurs early during foetal life. Progression into an overt tumour, however, typically first happens after puberty, where CIS cells transform into either a seminoma (SEM) or a nonseminoma (N-SEM). Here, we have compared the genome-wide gene expression of CIS cells to that of testicular SEM and a sample containing a mixture of N-SEM components, and analyse the data together with the previously published data on CIS. Genes showing expression in the SEM or N-SEM were selected, in order to identify gene expression markers associated with the progression of CIS cells. The identified markers were verified by reverse transcriptase-polymerase chain reaction and in situ hybridisation in a range of different TGCT samples. Verification showed some interpatient variation, but combined analysis of a range of the identified markers may discriminate TGCT samples as SEMs or N-SEMs. Of particular interest, we found that both DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta) and DNMT3L (DNA (cytosine-5-)-methyltransferase 3 like) were overexpressed in the N-SEMs, indicating the epigenetic differences between N-SEMs and classical SEM.

AB - The carcinoma in situ (CIS) cell is the common precursor of nearly all testicular germ cell tumours (TGCT). In a previous study, we examined the gene expression profile of CIS cells and found many features common to embryonic stem cells indicating that initiation of neoplastic transformation into CIS occurs early during foetal life. Progression into an overt tumour, however, typically first happens after puberty, where CIS cells transform into either a seminoma (SEM) or a nonseminoma (N-SEM). Here, we have compared the genome-wide gene expression of CIS cells to that of testicular SEM and a sample containing a mixture of N-SEM components, and analyse the data together with the previously published data on CIS. Genes showing expression in the SEM or N-SEM were selected, in order to identify gene expression markers associated with the progression of CIS cells. The identified markers were verified by reverse transcriptase-polymerase chain reaction and in situ hybridisation in a range of different TGCT samples. Verification showed some interpatient variation, but combined analysis of a range of the identified markers may discriminate TGCT samples as SEMs or N-SEMs. Of particular interest, we found that both DNMT3B (DNA (cytosine-5-)-methyltransferase 3 beta) and DNMT3L (DNA (cytosine-5-)-methyltransferase 3 like) were overexpressed in the N-SEMs, indicating the epigenetic differences between N-SEMs and classical SEM.

KW - Carcinoma in situ

KW - DNMT3L

KW - Gene expression

KW - Nonseminoma

KW - Seminoma

KW - Testicular cancer

UR - http://www.scopus.com/inward/record.url?scp=20344386954&partnerID=8YFLogxK

U2 - 10.1038/sj.bjc.6602560

DO - 10.1038/sj.bjc.6602560

M3 - Journal article

C2 - 15856041

AN - SCOPUS:20344386954

VL - 92

SP - 1934

EP - 1941

JO - The British journal of cancer. Supplement

JF - The British journal of cancer. Supplement

SN - 0007-0920

IS - 10

ER -

ID: 284207916