Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis

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Standard

Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis. / Nielsen, J. E.; Hansen, M. A.; Jørgensen, M.; Tanaka, Masami; Almstrup, K.; Skakkebæk, N. E.; Leffers, Henrik.

In: European Journal of Histochemistry, Vol. 47, No. 3, 2003, p. 215-222.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, JE, Hansen, MA, Jørgensen, M, Tanaka, M, Almstrup, K, Skakkebæk, NE & Leffers, H 2003, 'Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis', European Journal of Histochemistry, vol. 47, no. 3, pp. 215-222. https://doi.org/10.4081/830

APA

Nielsen, J. E., Hansen, M. A., Jørgensen, M., Tanaka, M., Almstrup, K., Skakkebæk, N. E., & Leffers, H. (2003). Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis. European Journal of Histochemistry, 47(3), 215-222. https://doi.org/10.4081/830

Vancouver

Nielsen JE, Hansen MA, Jørgensen M, Tanaka M, Almstrup K, Skakkebæk NE et al. Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis. European Journal of Histochemistry. 2003;47(3):215-222. https://doi.org/10.4081/830

Author

Nielsen, J. E. ; Hansen, M. A. ; Jørgensen, M. ; Tanaka, Masami ; Almstrup, K. ; Skakkebæk, N. E. ; Leffers, Henrik. / Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis. In: European Journal of Histochemistry. 2003 ; Vol. 47, No. 3. pp. 215-222.

Bibtex

@article{2d7f8dfd3f5647bca26860a4a38610d1,
title = "Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis",
abstract = "LANCL1 (LanC-like protein 1) is related to the bacterial LanC (lanthionine synthetase C) family, which is involved in the biosynthesis of antimicrobial peptides. Highest expression levels of LANCL1 are found in testes and brain, two organs that exist behind blood-tissue barriers. In the mouse, the establishment of an impermeable blood-testis barrier occurs between day 10-16 post natal (pn). Differential display analysis showed that the expression level of LANCL1 mRNA in mouse testes was very low until day 16 pn, but increased gradually from day 16 pn to reach a maximum on days 22-24 pn followed by a slight reduction from day 26 pn to adult animals. Thus, the expression of LANCL1 mRNA is initiated following the establishment of the blood-testis barrier. In situ hybridisation revealed that LANCL1 mRNA was induced in diplotene spermatocytes, which appear for the first time in mouse testes between days 18 and 20 pn, verifying the expression profile determined by differential display. LANCL1 mRNA level remained high in the meiotic division phase and in early round spermatids, but was down regulated in elongating spermatids and it was undetectable in step 9 elongating spermatids in stage IX (as defined by Russel et al., 1990). The steady decrease in expression level from round spermatids in stage I to elongating spermatids in stage IX suggested that LANCL1 mRNA was not transcribed in spermatids. LANCL1 expression in rat testes was initiated already in pachytene spermatocytes in stage IX, but otherwise similar to mouse.",
keywords = "Blood-testis barrier, In situ hybridization, LANCL1, Spermatogenesis",
author = "Nielsen, {J. E.} and Hansen, {M. A.} and M. J{\o}rgensen and Masami Tanaka and K. Almstrup and Skakkeb{\ae}k, {N. E.} and Henrik Leffers",
year = "2003",
doi = "10.4081/830",
language = "English",
volume = "47",
pages = "215--222",
journal = "European Journal of Histochemistry",
issn = "1121-760X",
publisher = "PagePress Publications",
number = "3",

}

RIS

TY - JOUR

T1 - Germ cell differentiation-dependent and stage-specific expression of LANCL1 in rodent testis

AU - Nielsen, J. E.

AU - Hansen, M. A.

AU - Jørgensen, M.

AU - Tanaka, Masami

AU - Almstrup, K.

AU - Skakkebæk, N. E.

AU - Leffers, Henrik

PY - 2003

Y1 - 2003

N2 - LANCL1 (LanC-like protein 1) is related to the bacterial LanC (lanthionine synthetase C) family, which is involved in the biosynthesis of antimicrobial peptides. Highest expression levels of LANCL1 are found in testes and brain, two organs that exist behind blood-tissue barriers. In the mouse, the establishment of an impermeable blood-testis barrier occurs between day 10-16 post natal (pn). Differential display analysis showed that the expression level of LANCL1 mRNA in mouse testes was very low until day 16 pn, but increased gradually from day 16 pn to reach a maximum on days 22-24 pn followed by a slight reduction from day 26 pn to adult animals. Thus, the expression of LANCL1 mRNA is initiated following the establishment of the blood-testis barrier. In situ hybridisation revealed that LANCL1 mRNA was induced in diplotene spermatocytes, which appear for the first time in mouse testes between days 18 and 20 pn, verifying the expression profile determined by differential display. LANCL1 mRNA level remained high in the meiotic division phase and in early round spermatids, but was down regulated in elongating spermatids and it was undetectable in step 9 elongating spermatids in stage IX (as defined by Russel et al., 1990). The steady decrease in expression level from round spermatids in stage I to elongating spermatids in stage IX suggested that LANCL1 mRNA was not transcribed in spermatids. LANCL1 expression in rat testes was initiated already in pachytene spermatocytes in stage IX, but otherwise similar to mouse.

AB - LANCL1 (LanC-like protein 1) is related to the bacterial LanC (lanthionine synthetase C) family, which is involved in the biosynthesis of antimicrobial peptides. Highest expression levels of LANCL1 are found in testes and brain, two organs that exist behind blood-tissue barriers. In the mouse, the establishment of an impermeable blood-testis barrier occurs between day 10-16 post natal (pn). Differential display analysis showed that the expression level of LANCL1 mRNA in mouse testes was very low until day 16 pn, but increased gradually from day 16 pn to reach a maximum on days 22-24 pn followed by a slight reduction from day 26 pn to adult animals. Thus, the expression of LANCL1 mRNA is initiated following the establishment of the blood-testis barrier. In situ hybridisation revealed that LANCL1 mRNA was induced in diplotene spermatocytes, which appear for the first time in mouse testes between days 18 and 20 pn, verifying the expression profile determined by differential display. LANCL1 mRNA level remained high in the meiotic division phase and in early round spermatids, but was down regulated in elongating spermatids and it was undetectable in step 9 elongating spermatids in stage IX (as defined by Russel et al., 1990). The steady decrease in expression level from round spermatids in stage I to elongating spermatids in stage IX suggested that LANCL1 mRNA was not transcribed in spermatids. LANCL1 expression in rat testes was initiated already in pachytene spermatocytes in stage IX, but otherwise similar to mouse.

KW - Blood-testis barrier

KW - In situ hybridization

KW - LANCL1

KW - Spermatogenesis

UR - http://www.scopus.com/inward/record.url?scp=0041334197&partnerID=8YFLogxK

U2 - 10.4081/830

DO - 10.4081/830

M3 - Journal article

C2 - 14514412

AN - SCOPUS:0041334197

VL - 47

SP - 215

EP - 222

JO - European Journal of Histochemistry

JF - European Journal of Histochemistry

SN - 1121-760X

IS - 3

ER -

ID: 284207317