Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm. / Rehfeld, Anders; Egeberg Palme, Dorte Louise; Almstrup, Kristian; Juul, Anders; Skakkebaek, Niels Erik.

In: Journal of visualized experiments : JoVE, No. 145, e59212, 01.03.2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rehfeld, A, Egeberg Palme, DL, Almstrup, K, Juul, A & Skakkebaek, NE 2019, 'Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm', Journal of visualized experiments : JoVE, no. 145, e59212. https://doi.org/10.3791/59212

APA

Rehfeld, A., Egeberg Palme, D. L., Almstrup, K., Juul, A., & Skakkebaek, N. E. (2019). Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm. Journal of visualized experiments : JoVE, (145), [e59212]. https://doi.org/10.3791/59212

Vancouver

Rehfeld A, Egeberg Palme DL, Almstrup K, Juul A, Skakkebaek NE. Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm. Journal of visualized experiments : JoVE. 2019 Mar 1;(145). e59212. https://doi.org/10.3791/59212

Author

Rehfeld, Anders ; Egeberg Palme, Dorte Louise ; Almstrup, Kristian ; Juul, Anders ; Skakkebaek, Niels Erik. / Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm. In: Journal of visualized experiments : JoVE. 2019 ; No. 145.

Bibtex

@article{e00839265c8d4c28b016aa76043f5176,
title = "Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm",
abstract = "Ca2+-signaling is essential to normal sperm cell function and male fertility. Similarly, the acrosome reaction is vital for the ability of a human sperm cell to penetrate the zona pellucida and fertilize the egg. It is therefore of great interest to test compounds (e.g., environmental chemicals or drug candidates) for their effect on Ca2+-signaling and acrosome reaction in human sperm either to examine the potential adverse effects on human sperm cell function or to investigate a possible role as a contraceptive. Here, two medium-throughput assays are described: 1) a fluorescence-based assay for assessment of effects on Ca2+-signaling in human sperm, and 2) an image cytometric assay for assessment of acrosome reaction in human sperm. These assays can be used to screen a large number of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm. Furthermore, the assays can be used to generate highly specific dose-response curves of individual compounds, determine potential additivity/synergism for two or more compounds, and to study the pharmacological mode of action through competitive inhibition experiments with CatSper inhibitors.",
keywords = "Acrosome Reaction, Animals, Biological Assay/methods, Calcium/metabolism, Calcium Signaling, Fluorescence, Fluorometry, Humans, Male, Semen/physiology, Spermatozoa/physiology",
author = "Anders Rehfeld and {Egeberg Palme}, {Dorte Louise} and Kristian Almstrup and Anders Juul and Skakkebaek, {Niels Erik}",
year = "2019",
month = mar,
day = "1",
doi = "10.3791/59212",
language = "English",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "Journal of Visualized Experiments",
number = "145",

}

RIS

TY - JOUR

T1 - Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

AU - Rehfeld, Anders

AU - Egeberg Palme, Dorte Louise

AU - Almstrup, Kristian

AU - Juul, Anders

AU - Skakkebaek, Niels Erik

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Ca2+-signaling is essential to normal sperm cell function and male fertility. Similarly, the acrosome reaction is vital for the ability of a human sperm cell to penetrate the zona pellucida and fertilize the egg. It is therefore of great interest to test compounds (e.g., environmental chemicals or drug candidates) for their effect on Ca2+-signaling and acrosome reaction in human sperm either to examine the potential adverse effects on human sperm cell function or to investigate a possible role as a contraceptive. Here, two medium-throughput assays are described: 1) a fluorescence-based assay for assessment of effects on Ca2+-signaling in human sperm, and 2) an image cytometric assay for assessment of acrosome reaction in human sperm. These assays can be used to screen a large number of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm. Furthermore, the assays can be used to generate highly specific dose-response curves of individual compounds, determine potential additivity/synergism for two or more compounds, and to study the pharmacological mode of action through competitive inhibition experiments with CatSper inhibitors.

AB - Ca2+-signaling is essential to normal sperm cell function and male fertility. Similarly, the acrosome reaction is vital for the ability of a human sperm cell to penetrate the zona pellucida and fertilize the egg. It is therefore of great interest to test compounds (e.g., environmental chemicals or drug candidates) for their effect on Ca2+-signaling and acrosome reaction in human sperm either to examine the potential adverse effects on human sperm cell function or to investigate a possible role as a contraceptive. Here, two medium-throughput assays are described: 1) a fluorescence-based assay for assessment of effects on Ca2+-signaling in human sperm, and 2) an image cytometric assay for assessment of acrosome reaction in human sperm. These assays can be used to screen a large number of compounds for effects on Ca2+-signaling and acrosome reaction in human sperm. Furthermore, the assays can be used to generate highly specific dose-response curves of individual compounds, determine potential additivity/synergism for two or more compounds, and to study the pharmacological mode of action through competitive inhibition experiments with CatSper inhibitors.

KW - Acrosome Reaction

KW - Animals

KW - Biological Assay/methods

KW - Calcium/metabolism

KW - Calcium Signaling

KW - Fluorescence

KW - Fluorometry

KW - Humans

KW - Male

KW - Semen/physiology

KW - Spermatozoa/physiology

U2 - 10.3791/59212

DO - 10.3791/59212

M3 - Journal article

C2 - 30882783

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 145

M1 - e59212

ER -

ID: 235532228