Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
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Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies. / Domljanovic, Ivana; Carstens, Annika; Okholm, Anders; Kjems, Jørgen; Nielsen, Christoffer Tandrup; Heegaard, Niels H H; Astakhova, Kira.
In: Scientific Reports, Vol. 7, 1925, 2017.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies
AU - Domljanovic, Ivana
AU - Carstens, Annika
AU - Okholm, Anders
AU - Kjems, Jørgen
AU - Nielsen, Christoffer Tandrup
AU - Heegaard, Niels H H
AU - Astakhova, Kira
PY - 2017
Y1 - 2017
N2 - To date, there are multiple assays developed that detect and quantify antibodies in biofluids. Nevertheless, there is still a lack of simple approaches that specifically detect autoimmune antibodies to double-stranded DNA. Herein we investigate the potential of novel nucleic acid complexes as targets for these antibodies. This is done in a simple, rapid and specific immunofluorescence assay. Specifically, employing 3D nanostructures (DNA origami), we present a new approach in the detection and study of human antibodies to DNA. We demonstrate the detection of anti-DNA antibodies that are characteristic of systemic lupus erythematosus, a chronic autoimmune disease with multiple manifestations. We tested the most potent non-covalent pairs of DNA and fluorescent dyes. Several complexes showed specific recognition of autoimmune antibodies in human samples of lupus patients using a simple one-step immunofluorescence method. This makes the novel assay developed herein a promising tool for research and point-of-care monitoring of anti-DNA antibodies. Using this method, we for the first time experimentally confirm that the disease-specific autoimmune antibodies are sensitive to the 3D structure of nucleic acids and not only to the nucleotide sequence, as was previously thought.
AB - To date, there are multiple assays developed that detect and quantify antibodies in biofluids. Nevertheless, there is still a lack of simple approaches that specifically detect autoimmune antibodies to double-stranded DNA. Herein we investigate the potential of novel nucleic acid complexes as targets for these antibodies. This is done in a simple, rapid and specific immunofluorescence assay. Specifically, employing 3D nanostructures (DNA origami), we present a new approach in the detection and study of human antibodies to DNA. We demonstrate the detection of anti-DNA antibodies that are characteristic of systemic lupus erythematosus, a chronic autoimmune disease with multiple manifestations. We tested the most potent non-covalent pairs of DNA and fluorescent dyes. Several complexes showed specific recognition of autoimmune antibodies in human samples of lupus patients using a simple one-step immunofluorescence method. This makes the novel assay developed herein a promising tool for research and point-of-care monitoring of anti-DNA antibodies. Using this method, we for the first time experimentally confirm that the disease-specific autoimmune antibodies are sensitive to the 3D structure of nucleic acids and not only to the nucleotide sequence, as was previously thought.
U2 - 10.1038/s41598-017-02214-0
DO - 10.1038/s41598-017-02214-0
M3 - Journal article
C2 - 28507286
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 1925
ER -
ID: 196044311