TY - JOUR

T1 - Effects of glutamine on the immune system

T2 - Influence of muscular exercise and HIV infection

AU - Rohde, T.

AU - Ullum, H.

AU - Palmo Rasmussen, J.

AU - Kristensen, J. H.

AU - Newsholme, E.

AU - Pedersen, B. K.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Glutamine increased the proliferative response and the lymphokine- activated killer cell activity of blood mononuclear cells isolated from normal healthy subjects (n = 6) in a dose-dependent manner, with optimum at 0.3-1.0 mM. The relative fraction of CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells was not changed by glutamine at a concentration of 0.6 mM, except in the phytohemagglutinin-stimulated proliferation experiment where the fraction of CD4+, and therefore CD3+ cells, increased. The natural killer cell activity was not influenced by glutamine. Human immunodeficiency virus (HIV)-seropositive subjects (n = 8) who performed concentric bicycle exercise for 1 h at 75% of maximal O2 consumption had an overall lower phytohemagglutinin-stimulated proliferative response, compared with the HIV- seronegative control group (n = 7). The proliferation during exercise was lower in both the HIV-seropositive and the HIV-seronegative group. Addition of glutamine in vitro did not normalize the lower proliferation in the HIV- seropositive group or the attenuated proliferation seen during exercise in both groups.

AB - Glutamine increased the proliferative response and the lymphokine- activated killer cell activity of blood mononuclear cells isolated from normal healthy subjects (n = 6) in a dose-dependent manner, with optimum at 0.3-1.0 mM. The relative fraction of CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells was not changed by glutamine at a concentration of 0.6 mM, except in the phytohemagglutinin-stimulated proliferation experiment where the fraction of CD4+, and therefore CD3+ cells, increased. The natural killer cell activity was not influenced by glutamine. Human immunodeficiency virus (HIV)-seropositive subjects (n = 8) who performed concentric bicycle exercise for 1 h at 75% of maximal O2 consumption had an overall lower phytohemagglutinin-stimulated proliferative response, compared with the HIV- seronegative control group (n = 7). The proliferation during exercise was lower in both the HIV-seropositive and the HIV-seronegative group. Addition of glutamine in vitro did not normalize the lower proliferation in the HIV- seropositive group or the attenuated proliferation seen during exercise in both groups.

KW - exercise

KW - glutamine

KW - human immunodeficiency virus

KW - immune system

KW - lymphocyte subpopulations

KW - lymphocyte-proliferative response

KW - lymphokine- activated killer cells

KW - natural killer cells

U2 - 10.1152/jappl.1995.79.1.146

DO - 10.1152/jappl.1995.79.1.146

M3 - Journal article

C2 - 7559212

AN - SCOPUS:0029102045

VL - 79

SP - 146

EP - 150

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 1

ER -